Nucleotide sequence analysis of the long terminal repeat of human T-cell leukemia virus type II.
Nucleotide sequence analysis of the long terminal repeat of human T-cell leukemia virus type II.
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人 T 细胞白血病病毒 II 型长末端重复序列的核苷酸序列分析。
DOI：
10.1073/pnas.81.4.1079
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发表时间：
1984
影响因子：
11.1
通讯作者：
Chen,IS
中科院分区：
文献类型：
--
作者：
Shimotohno,K;Golde,DW;Miwa,M;Sugimura,T;Chen,IS
测定了人T细胞白血病病毒II型（HTLV-II）长末端重复序列（LTR）及其周围区域的核苷酸序列。我们的研究结果显示了以下结构特征：（i）LTR长763个碱基对（bp），由314 +/- 1 bp的U3区，248 +/- 1 bp的R区和201 bp的U 5区组成;（ii）LTR的末端核苷酸形成T-G的反向重复序列。C-A;（iii）6-bp的细胞序列侧翼的前病毒的直接重复序列存在;和（iv）病毒RNA合成的起始或终止的推定功能信号进行了鉴定。HTLV-II LTR序列与先前发表的成人T细胞白血病病毒（ATLV; HTLV-I）的序列比较表明，LTR是不同的。HTLV-II和ATLV之间的一些小区域是保守的，包括对转录重要的序列和在U3中重复三次的21个核苷酸的序列。这个21 bp的重复序列可能在调节淋巴样细胞中的病毒转录中起重要作用。
The nucleotide sequence of the human T-cell leukemia virus type II (HTLV-II) long terminal repeat (LTR) and its surrounding regions were determined. Our results show the following structural features: (i) the LTR is 763 base pairs (bp) in length and consists of 314 +/- 1 bp of region U3, 248 +/- 1 bp of region R, and 201 bp of region U5; (ii) the terminal nucleotides in the LTR form an inverted repeat of T-G.....C-A; (iii) 6-bp direct repeats of cellular sequences flanking the provirus were present; and (iv) the putative functional signals for initiation or termination of viral RNA synthesis were identified. Comparison of the HTLV-II LTR sequence with that previously published for adult T-cell leukemia virus (ATLV; HTLV-I) shows that the LTRs are distinct. Some small regions are conserved between HTLV-II and ATLV, involving sequences important for transcription and a sequence of 21 nucleotides repeated three times in U3. This 21-bp repeat may be important in regulating viral transcription in lymphoid cells.