Evaluation of polyelectrolyte complex-based scaffolds for mesenchymal stem cell therapy in cardiac ischemia treatment
Evaluation of polyelectrolyte complex-based scaffolds for mesenchymal stem cell therapy in cardiac ischemia treatment
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DOI：
10.1016/j.actbio.2013.10.027
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发表时间：
2014-02-01
影响因子：
9.7
通讯作者：
Fullana, Sophie Girod
中科院分区：
文献类型：
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作者：
Ceccaldi, Caroline;Bushkalova, Raya;Fullana, Sophie Girod
三维（3D）支架在基于干细胞的治疗中具有巨大的潜力。事实上，最近的结果表明，仿生支架可以提高细胞存活率，并促进损伤部位治疗细胞浓度的增加。这项工作的目的是工程的基础上，分别有益的效果藻酸盐和壳聚糖的原始聚合物支架。制剂由各种藻酸盐/壳聚糖比率制成以形成相反电荷的壳聚糖复合物（佩奇）。冷冻干燥后，所得基质呈现出高度互连的多孔微观结构和适于细胞培养的机械性能。体外评价表明它们与间充质星形细胞（MSC）增殖的相容性和它们维持旁分泌活性的能力。最后，在大鼠模型中诱发急性心肌梗死后，评价了聚合比为40/60的接种3D PEC支架的体内性能。心脏功能评价显示，与动物对照组相比，射血分数显著增加，新血管形成改善，纤维化减弱以及左心室扩张减少。这些结果提供了证据表明，由藻酸盐和壳聚糖制备的3D PEC支架为MSC的3D培养提供了有效的环境，并代表了组织工程的创新解决方案。(C)2013 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Three-dimensional (3D) scaffolds hold great potential for stem cell-based therapies. Indeed, recent results have shown that biomimetic scaffolds may enhance cell survival and promote an increase in the concentration of therapeutic cells at the injury site. The aim of this work was to engineer an original polymeric scaffold based on the respective beneficial effects of alginate and chitosan. Formulations were made from various alginate/chitosan ratios to form opposite-charge polyelectrolyte complexes (PECs). After freeze-drying, the resultant matrices presented a highly interconnected porous microstructure and mechanical properties suitable for cell culture. In vitro evaluation demonstrated their compatibility with mesenchymal stell cell (MSC) proliferation and their ability to maintain paracrine activity. Finally, the in vivo performance of seeded 3D PEC scaffolds with a polymeric ratio of 40/60 was evaluated after an acute myocardial infarction provoked in a rat model. Evaluation of cardiac function showed a significant increase in the ejection fraction, improved neovascularization, attenuated fibrosis as well as less left ventricular dilatation as compared to an animal control group. These results provide evidence that 3D PEC scaffolds prepared from alginate and chitosan offer an efficient environment for 3D culturing of MSCs and represent an innovative solution for tissue engineering. (C) 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.