Lateral density of receptor arrays in the membrane plane influences sensitivity of the E. coli chemotaxis response
Lateral density of receptor arrays in the membrane plane influences sensitivity of the E. coli chemotaxis response
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DOI：
10.1038/emboj.2011.77
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发表时间：
2011-05-04
期刊：
影响因子：
11.4
通讯作者：
Subramaniam, Sriram
中科院分区：
文献类型：
--
作者：
Khursigara, Cezar M.;Lan, Ganhui;Subramaniam, Sriram
在趋化性细菌中，跨膜化学感受器CheA和CheW形成趋化性感觉器的核心信号复合物。这些复合物在细胞质膜中组织成延伸阵列，其允许细菌通过协同变构反应来响应细胞外配体浓度的变化，所述协同变构反应导致由配体结合诱导的信号的显著放大。在这里，我们结合冷冻电子断层扫描研究的三维空间结构的化学感受器阵列在完整的大肠杆菌。大肠杆菌细胞与计算建模，以开发一个预测模型的协同性和敏感性的趋化反应。使用荧光共振能量转移（FRET）显微镜实验测试的预测。我们的研究结果表明，部分有序的，空间扩展的化学感受器阵列的横向包装密度的变化可以调节细菌的趋化反应，并通过冷冻电子断层扫描的完整细胞所得到的阵列的分子组织的信息可以转化为可测试的，预测的计算模型的趋化反应。The EMBO Journal（2011）30，1719-1729. doi：10.1038/daj.2011.77; 2011年3月25日在线发布
In chemotactic bacteria, transmembrane chemoreceptors, CheA and CheW form the core signalling complex of the chemotaxis sensory apparatus. These complexes are organized in extended arrays in the cytoplasmic membrane that allow bacteria to respond to changes in concentration of extracellular ligands via a cooperative, allosteric response that leads to substantial amplification of the signal induced by ligand binding. Here, we have combined cryo-electron tomographic studies of the 3D spatial architecture of chemoreceptor arrays in intact E. coli cells with computational modelling to develop a predictive model for the cooperativity and sensitivity of the chemotaxis response. The predictions were tested experimentally using fluorescence resonance energy transfer (FRET) microscopy. Our results demonstrate that changes in lateral packing densities of the partially ordered, spatially extended chemoreceptor arrays can modulate the bacterial chemotaxis response, and that information about the molecular organization of the arrays derived by cryo-electron tomography of intact cells can be translated into testable, predictive computational models of the chemotaxis response. The EMBO Journal (2011) 30, 1719-1729. doi:10.1038/emboj.2011.77; Published online 25 March 2011