IMMUNOLOGICAL ABNORMALITIES IN HUMAN IMMUNODEFICIENCY VIRUS (HIV)-INFECTED ASYMPTOMATIC HOMOSEXUAL MEN - HIV AFFECTS THE IMMUNE-SYSTEM BEFORE CD4+ T-HELPER CELL DEPLETION OCCURS
IMMUNOLOGICAL ABNORMALITIES IN HUMAN IMMUNODEFICIENCY VIRUS (HIV)-INFECTED ASYMPTOMATIC HOMOSEXUAL MEN - HIV AFFECTS THE IMMUNE-SYSTEM BEFORE CD4+ T-HELPER CELL DEPLETION OCCURS
复制标题
DOI：
10.1172/jci113809
复制
发表时间：
1988-12-01
影响因子：
15.9
通讯作者：
SCHELLEKENS, PTA
中科院分区：
文献类型：
--
作者：
MIEDEMA, F;PETIT, AJC;SCHELLEKENS, PTA
为了研究持续HIV感染对免疫系统的影响，我们研究了14名无症状同性恋男性（CDC组II/III）的白细胞功能，这些男性至少两年血清阳性，但循环中的CD 4 + T细胞数量仍然正常。与年龄匹配的异性恋男性和HIV阴性的同性恋男性相比，来自血清阳性男性的CD 4+和CD 8 + T细胞表现出对抗CD 3单克隆抗体的增殖降低，并且在PWM驱动的正常供体B细胞分化中CD 4 + T辅助细胞活性降低。HIV感染者的单核细胞对CD 3单克隆抗体诱导的正常T细胞增殖的辅助功能降低。在HIV感染者的B细胞中观察到白细胞功能活动的最显著缺陷。14名血清反应阳性的男性中有13名的B细胞在存在足够的同种异体T辅助细胞活性的情况下对PWM反应不产生IG。这些发现表明，HIV在感染早期在T细胞、B细胞和抗原呈递细胞中诱导严重的免疫异常，然后CD 4 + T细胞数量开始下降。亚临床HIV感染患者的免疫功能受损可能对疫苗接种策略具有临床意义，特别是在HIV血清阳性率高的人群中使用活疫苗。
To investigate the effect of persistent HIV infection on the immune system, we studied leukocyte functions in 14 asymptomatic homosexual men (CDC group II/III) who were at least two years seropositive, but who still had normal numbers of circulating CD4+ T cells. Compared with age-matched heterosexual men and HIV-negative homosexual men, the CD4+ and CD8+ T cells from seropositive men showed decreased proliferation to anti-CD3 monoclonal antibody and decreased CD4+ T-helper activity on PWM-driven differentiation of normal donor B cells. Monocytes of HIV-infected homosexual men showed decreased accessory function on normal T cell proliferation induced by CD3 monoclonal antibody. The most striking defect in leukocyte functional activities was observed in the B cells of HIV-infected men. B cells of 13 out of 14 seropositive men failed to produce Ig in response to PWM in the presence of adequate allogeneic T-helper activity. These findings suggest that HIV induces severe immunological abnormalities in T cells, B cells, and antigen-presenting cells early in infection before CD4+ T cell numbers start to decline. Impaired immunological function in subclinically HIV-infected patients may have clinical implications for vaccination strategies, in particular the use of live vaccines in groups with a high prevalence of HIV seropositivity.