Photothermal Ablation of in Situ Renal Tumor by PEG-IR780-C13 Micelles and Near-Infrared Irradiation
Photothermal Ablation of in Situ Renal Tumor by PEG-IR780-C13 Micelles and Near-Infrared Irradiation
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PEG-IR780-C13胶束和近红外照射光热消融原位肾肿瘤
DOI：
10.1021/acs.molpharmaceut.5b00734
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发表时间：
2016-03-01
影响因子：
4.9
通讯作者：
Hu, Yiqiao
中科院分区：
文献类型：
--
作者：
Qiu, Xuefeng;Xu, Linfeng;Hu, Yiqiao
PEG-IR 780-C13胶束是一种新型的具有肿瘤靶向性的光热剂。本研究旨在通过原位肿瘤模型探讨PEG-IR 780-C13胶束结合近红外辐射热消融治疗肾肿瘤的可行性。此外，对正常肾组织的潜在热损伤进行了评价。PEG-IR 780-C13胶束旨在在全身递送后在肾肿瘤中积累。体外实验结果显示PEG-IR 780-C13胶束主要通过胞膜小窝介导的内吞作用被RENCA细胞摄取，并主要分布在晚期内体和溶酶体中。在近红外辐射下，PEG-IR 780-C13胶束在体外和体内均有效地产生热量，表现出有希望的光热治疗性质。PEG-IR 780-C13胶束的光热效应在体外可有效杀伤RENCA细胞，在体内可有效抑制原位肾癌的生长。同时，PEG-IR 780-C13胶束介导的光热治疗（PTT）仅对肿瘤部位周围的正常肾组织造成有限的损伤。我们的数据表明，PEG-IR 780-C13胶束介导的PTT可以产生肿瘤特异性热，以微创的方式破坏肾肿瘤，为肾肿瘤的热消融提供了一种新的策略。
PEG-IR780-C13 micelles have been demonstrated to be a novel photothermal agent with tumor-targeting property. This study was designed to explore the feasibility of applying PEG-IR780-C13 micelles and near-infrared (NIR) irradiation for thermal ablation of renal tumor by using an in situ tumor model. In addition, the potential thermal injury to normal renal tissue was evaluated. PEG-IR780-C13 micelles were intended to accumulate in renal tumor after systemic delivery. In vitro results revealed that PEG-IR780-C13 micelles were uptaken by RENCA cells mainly through caveola-mediated endocytosis and mainly distributed in late endosomes and lysosomes. Upon NIR irradiation, PEG-IR780-C13 micelles generated heat effectively both in vitro and in vivo, exhibiting a promising photothermal therapeutic property. The photothermal effect of PEG-IR780-C13 micelles could effectively destroy RENCA cells in vitro and adequately inhibit growth of in situ renal tumor in vivo. Meanwhile, PEG-IR780-C13 micelles mediated photothermal therapy (PTT) resulting in only limited injury to normal renal tissue surrounding tumor sites. Our data indicated that PEG-IR780-C13 micelles mediating PTT could generate tumor-specific heat for destruction of renal tumor in a minimally invasive way, providing a novel strategy for thermal ablation of renal tumor.