Detection of dietetically absorbed maize-derived microRNAs in pigs.
Detection of dietetically absorbed maize-derived microRNAs in pigs.
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猪体内饮食吸收的玉米来源 microRNA 的检测
DOI：
10.1038/s41598-017-00488-y
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发表时间：
2017-04-05
影响因子：
4.6
通讯作者：
Li M
中科院分区：
文献类型：
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作者：
Luo Y;Wang P;Wang X;Wang Y;Mu Z;Li Q;Fu Y;Xiao J;Li G;Ma Y;Gu Y;Jin L;Ma J;Tang Q;Jiang A;Li X;Li M
MicroRNA是一类在动植物转录后基因调控中起重要作用的小分子RNA。这些单链分子广泛分布在生物体中，影响基本的生物过程。有趣的是，最近的研究报道，饮食来源的植物miRNAs可以调节哺乳动物的基因表达，这些研究拓宽了我们对跨王国通信的看法。在本研究中，我们评估了含有熟玉米的食物饮食中的miRNA水平，发现植物miRNA在一定程度上抵抗苛刻的烹饪条件。将新鲜玉米饲喂猪后（7天），在猪的组织和血清中检测到玉米来源的miRNAs，并通过氧化反应证实了这些植物miRNAs的真实性。此外，体内和体外实验表明，玉米miRNAs可以通过胃肠道进入猪的血液。在猪细胞中，我们发现植物miRNAs很可能特异性靶向其内源性猪mRNAs，并以类似于哺乳动物miRNAs的方式影响基因表达。我们的研究结果表明，玉米衍生的miRNA可以穿过胃肠道并存在于猪体内，这些外源性miRNA具有调节哺乳动物基因表达的潜力。
MicroRNAs are a class of small RNAs that are important in post-transcriptional gene regulation in animals and plants. These single-stranded molecules are widely distributed in organisms and influence fundamental biological processes. Interestingly, recent studies have reported that diet-derived plant miRNAs could regulate mammalian gene expression, and these studies have broadened our view of cross-kingdom communication. In the present study, we evaluated miRNA levels in cooked maize-containing chow diets, and found that plant miRNAs were resistant to the harsh cooking conditions to a certain extent. After feeding fresh maize to pigs (7 days), maize-derived miRNAs could be detected in porcine tissues and serum, and the authenticity of these plant miRNAs was confirmed by using oxidization reactions. Furthermore,in vivoandin vitroexperiments demonstrated that dietary maize miRNAs could cross the gastrointestinal tract and enter the porcine bloodstream. In the porcine cells, we found that plant miRNAs are very likely to specifically target their endogenous porcine mRNAs and influence gene expression in a fashion similar to that of mammalian miRNAs. Our results indicate that maize-derived miRNAs can cross the gastrointestinal tract and present in pigs, and these exogenous miRNAs have the potential to regulate mammalian gene expression.