Characterization of two non-homoeologous nuclear genes encoding mitochondrial alternative oxidase in common wheat
Characterization of two non-homoeologous nuclear genes encoding mitochondrial alternative oxidase in common wheat
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DOI：
10.1266/ggs.77.81
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发表时间：
2002-04-01
影响因子：
1.1
通讯作者：
Nakamura, C
中科院分区：
文献类型：
--
作者：
Takumi, S;Tomioka, M;Nakamura, C
线粒体替代氧化酶 (AOX) 是负责氰化物不敏感和水杨异羟肟酸敏感呼吸的末端氧化酶。我们从普通小麦（Triticum aestivum L.）中分离出了两个编码 AOX 蛋白的非同源基因（Waox1a 和 Waox1c）。这两个基因与水稻 AOX1a 和 AOX1c 是直系同源的，并且它们的外显子/内含子结构是保守的，就像大多数其他植物 AOX 基因一样。 Southern blot分析表明Waox1a和Waox1c至少位于三个同源位点，并且普通小麦基因组中还存在同源性较低的AOX基因。 Waox1a 和 Waox1c 位点分别分配给同源的 2 号和 6 号染色体组。 Waox1a 和 Waox1c 转录物的稳态水平在冷应激下增加，而只有 Waox1a 的转录物水平在氰化物处理下增加。
Mitochondrial alternative oxidase (AOX) is the terminal oxidase responsible for cyanide-insensitive and salicylhydroxamic acid-sensitive respiration. We have isolated two non-homoeologous genes (Waox1a and Waox1c) encoding AOX proteins from common wheat (Triticum aestivum L.). These two genes were orthologous to rice AOX1a and AOX1c, and their exon/intron structure was conserved, as it is in most other plant AOX genes. Southern blot analysis indicated that both Waox1a and Waox1c were located in at least three homoeologous loci and that additional AOX genes with lower homology were present in the genome of common wheat. The Waox1a and Waox1c loci were respectively assigned to the homoeologous group 2 and 6 chromosomes. The steady-state level of Waox1a and Waox1c transcripts increased under cold stress, while only that of Waox1a was increased by cyanide treatment.