NMDA and non-NMDA receptors mediate visual responses of neurons in the cat's lateral geniculate nucleus.
NMDA and non-NMDA receptors mediate visual responses of neurons in the cat's lateral geniculate nucleus.
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DOI：
10.1152/jn.1991.66.2.414
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发表时间：
1991-08
影响因子：
2.5
通讯作者：
Y. H. Kwon;M. Esguerra;M. Sur
中科院分区：
文献类型：
--
作者：
Y. H. Kwon;M. Esguerra;M. Sur
1.我们研究了兴奋性氨基酸受体特异性拮抗剂对猫外侧膝状体（LGN）A层或A1层细胞视觉反应的影响。2.细胞被分类为中心或偏离中心，X或Y，滞后或非滞后。研究拮抗剂的作用，同时用覆盖感受野中心的适当对比度的斑点刺激细胞。3. N-甲基-D-天冬氨酸（NMDA）受体拮抗剂D-2-氨基-5-膦酰基戊酸（D-APV）和3-（+/-）-2-羧基哌嗪-4-基）-丙基-1-膦酸（CPP），当以特异性拮抗NMDA诱导的反应但不拮抗红藻氨酸诱导的反应的剂量进行离子导入时，降低了LGN中所有细胞类型的反应，包括X和Y细胞、滞后和非滞后细胞，以及中心和偏离中心的细胞。4.非NMDA受体拮抗剂6-氰基-7-硝基喹喔啉-2，3-二酮（CNQX），当应用剂量特异性拮抗红藻氨酸诱导的反应，但不是NMDA诱导的反应，也减少了视觉反应的每种细胞类型的LGN。5.定量分析了D-APV和CNQX对LGN细胞的作用。D-APV降低滞后细胞的反应在更大程度上比nonlagged细胞的反应。CNQX降低滞后和nonlagged细胞的反应，以类似的程度。D-APV或CNQX对X和Y细胞或对中心和偏离中心细胞的作用没有差异。6.我们分析了拮抗剂对反应的单独组分的影响，包括包含前100 ms反应的早期组分和包含接下来300 ms反应的晚期组分。D-APV降低滞后细胞反应的晚期成分在更大程度上比相同细胞的早期成分或非滞后细胞的早期或晚期成分。CNQX对所有细胞类型的两种反应组分具有几乎相同的作用。7.这些数据表明，NMDA受体和非NMDA受体作出类似的贡献，在LGN的上和偏离中心和X和Y细胞的反应。滞后和nonlagged细胞不分化的非NMDA受体的贡献，他们的视觉反应。NMDA受体对滞后细胞反应的较大贡献与这些受体对滞后细胞晚期反应成分的较大贡献一致。（250字处删节）
1. We have examined the effects of iontophoresing specific antagonists to excitatory amino acid receptors on the visual responses of cells in lamina A or A1 of the cat's lateral geniculate nucleus (LGN). 2. Cells were classified as On- or Off-center, X or Y, and lagged or nonlagged. The effects of antagonists were studied while cells were stimulated with spots of the appropriate contrast covering the receptive-field center. 3. The N-methyl-D-aspartate (NMDA) receptor antagonists D-2-amino-5-phosphonovaleric acid (D-APV) and 3-(+/-)-2-carboxypiperazin-4-yl)- propyl-1-phosphonic acid (CPP), when iontophoresed at doses that specifically antagonized NMDA-induced responses but not kainate-induced responses, reduced the responses of all cell types in the LGN, including X and Y cells, lagged and nonlagged cells, and On- and Off-center cells. 4. The non-NMDA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), when applied at doses that specifically antagonized kainate-induced responses but not NMDA-induced responses, also reduced the visual responses of each of the cell types in the LGN. 5. We analyzed quantitatively the effects of D-APV and CNQX on LGN cells. D-APV reduced the responses of lagged cells to a greater extent than the responses of nonlagged cells. CNQX reduced the responses of lagged and nonlagged cells to a similar extent. There was no difference in the effect of D-APV or of CNQX on X and Y cells or on On- and Off-center cells. 6. We analyzed the effects of the antagonists on separate components of responses, including an early component comprising the first 100 ms of response and a late component comprising the next 300 ms of response. D-APV reduced the late component of lagged cell responses to a greater extent than either the early component of the same cells or the early or late component of nonlagged cells. CNQX had nearly equivalent effects on both response components of all cell types. 7. These data indicate that NMDA and non-NMDA receptors make similar contributions to the responses of On- and Off-center and X and Y cells in the LGN. Lagged and nonlagged cells are not differentiated with respect to the contribution of non-NMDA receptors to their visual responses. The greater contribution of NMDA receptors to the responses of lagged cells is consistent with the large contribution made by these receptors to the late response components of lagged cells.(ABSTRACT TRUNCATED AT 250 WORDS)