Methanogenic crude oil degradation induced by an exogenous microbial community and nutrient injections
Methanogenic crude oil degradation induced by an exogenous microbial community and nutrient injections
复制标题
DOI：
10.1016/j.petrol.2021.108458
复制
发表时间：
2021-01
影响因子：
--
通讯作者：
K. Suda;M. Ikarashi;H. Tamaki;Satoshi Tamazawa;S. Sakata;Maeda Haruo;Y. Kamagata;M. Kaneko;
中科院分区：
文献类型：
--
作者：
K. Suda;M. Ikarashi;H. Tamaki;Satoshi Tamazawa;S. Sakata;Maeda Haruo;Y. Kamagata;M. Kaneko;
微生物降解原油产甲烷的潜力作为微生物强化能量回收（梅尔）技术的基础而备受关注。在这里，我们进行了模拟地下油藏的原位温度和压力条件的室内培养实验，以评估生物强化和刺激梅尔的有效性。利用我们的高温高压培养系统，我们成功地从日本山形油田获得了一个能够降解产甲烷原油的微生物群落。为了评估生物强化的潜力，我们将来自山形的微生物培养物接种到来自秋田油藏的生产水中，其中土著微生物群落不能降解原油。我们观察到与甲苯降解相关的甲烷产生，表明生物强化可能是成功的。我们还评估了生物刺激的潜力，即使用山形油藏样品补充不同营养物对原油降解的影响。有趣的是，添加特定的酵母提取物（YES）导致甲烷产量显着提高与长链正烷烃以及甲苯的降解。我们进一步评估了通过将来自Yamagata的微生物培养物与有效YE一起注入秋田生产水中来组合生物强化和刺激的有效性，并发现在孵育期间大量甲烷产生与甲苯和正烷烃降解相结合。总之，我们的研究结果表明，这里展示的生物增强和刺激策略可能是一个强大的工具，从石油储层中回收能源，无论是否有先天原油降解。
Microbial potential of crude oil degradation coupled to methane production has attracted much attention as a basis for microbial enhanced energy recovery (MEER) technology. Here we performed laboratory incubation experiments mimickingin situtemperature and pressure conditions of subsurface oil reservoirs to evaluate the effectiveness of biological augmentation and stimulation for MEER. Using our high temperature and pressure incubation system, we successfully obtained a microbial community capable of methanogenic crude oil degradation from the Yamagata oil reservoir in Japan. To evaluate the potential of biological augmentation, we inoculated the microbial culture from Yamagata into production water from the Akita oil reservoir in which the indigenous microbial community cannot degrade crude oil. We observed methane production associated with toluene degradation, indicating that biological augmentation could be successful. We also evaluated the potential of biological stimulation, i.e. the effects of supplementing with different nutrients on crude oil degradation using the Yamagata oil reservoir sample. Intriguingly, addition of specific yeast extracts (YEs) resulted in notably enhanced methane production associated with the degradation of longer-chainn-alkanes as well as toluene. We further assessed the effectiveness of combining the biological augmentation and stimulation by inoculating the microbial culture from Yamagata together with the effective YE into the Akita production water, and found a large amount of methane production coupled to toluene andn-alkane degradation during the incubation. Taken together, our results suggest that the bio-augmentation and stimulation strategies demonstrated here could be a powerful tool for energy recovery from petroleum reservoirs both with and without innate crude oil degradation.