Membrane-active peptides: binding, translocation, and flux in lipid vesicles.
Membrane-active peptides: binding, translocation, and flux in lipid vesicles.
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DOI：
10.1016/j.bbamem.2014.04.014
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发表时间：
2014-09
期刊：
影响因子：
--
通讯作者：
Almeida PF
中科院分区：
文献类型：
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作者：
Almeida PF
最近，新的和改进的方法已被开发来测量跨脂质双层膜的膜活性肽（抗微生物，细胞溶解，和两亲性细胞穿透肽）的易位。的假设，跨脂质双层的膜活性肽的易位是由插入的肽到双层的吉布斯能被重新检查在新的实验测试的光。最初的假设及其动机首先被重新审视，检查它产生的一些具体的预测和初步测试的结果。易位被理解为需要两个先前的步骤：结合和插入膜中。肽结合膜的问题，其预测，测量和计算得到解决。特别注意理解膜活性肽功能中需要两亲结构的原因。然后检查插入到膜中。疏水性尺度进行了比较，并讨论了它们对计算的影响。易位和分级或全或无肽诱导的流量或进入脂质囊泡之间的关系也被认为是。最后，最近的工作易位检查，无论是实验和分子动力学模拟。
Recently, new and improved methods have been developed to measure translocation of membrane-active peptides (antimicrobial, cytolytic, and amphipathic cell-penetrating peptides) across lipid bilayer membranes. The hypothesis that translocation of membrane-active peptides across a lipid bilayer is determined by the Gibbs energy of insertion of the peptide into the bilayer is re-examined in the light of new experimental tests. The original hypothesis and its motivation are first revisited, examining some specific predictions it generated and the results of initial tests. Translocation is understood as requiring two previous steps: binding and insertion in the membrane. The problem of peptide binding to membranes, its prediction, measurement, and calculation are addressed. Particular attention is given to understanding the reason for the need for amphipathic structures in the function of membrane-active peptides. Insertion into the membrane is then examined. Hydrophobicity scales are compared, and their influence on calculations is discussed. The relation between translocation and graded or all-or-none peptide-induced flux from or into lipid vesicles is also considered. Finally, the most recent work on translocation is examined, both experimental and from molecular dynamics simulations.