ESCHERICHIA-COLI INVASION OF BRAIN MICROVASCULAR ENDOTHELIAL-CELLS IN-VITRO AND IN-VIVO - MOLECULAR-CLONING AND CHARACTERIZATION OF INVASION GENE IBE10
ESCHERICHIA-COLI INVASION OF BRAIN MICROVASCULAR ENDOTHELIAL-CELLS IN-VITRO AND IN-VIVO - MOLECULAR-CLONING AND CHARACTERIZATION OF INVASION GENE IBE10
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DOI：
10.1128/iai.63.11.4470-4475.1995
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发表时间：
1995-11-01
影响因子：
3.1
通讯作者：
KIM, KS
中科院分区：
文献类型：
--
作者：
HUANG, SH;WASS, C;KIM, KS
大多数新生儿大肠埃希氏菌脑膜炎是由血源性传播引起的，但目前尚不清楚循环中的大肠杆菌是如何跨越血脑屏障的。为了鉴定导致中枢神经系统(CNS)侵袭的大肠杆菌结构，用E.ColiK1株RS218(O18：K1：H7)的侵袭性脑脊液分离株进行TnPhoA突变，并检测其在脑微血管内皮细胞(BMEC)中的非侵袭性能力。
Most cases of neonatal Escherichia coli meningitis develop as a result of hematogenous spread, but it is not clear how circulating E. coli crosses the blood-brain barrier, In an attempt to identify E. coli structures contributing to invasion into the central nervous system (CNS), TnphoA mutagenesis was performed with an invasive CSF isolate of E. coli K1 strain RS218 (O18:K1:H7), and TnphoA mutants were examined for their noninvasive capability in brain microvascular endothelial cells (BMEC), The noninvasive mutants exhibited the invasive ability of