Engineered protein switches for exogenous control of gene expression
Engineered protein switches for exogenous control of gene expression
复制标题
用于基因表达外源控制的工程蛋白质开关
DOI：
10.1042/bst20200441
复制
发表时间：
2020
影响因子：
3.9
通讯作者：
Ostermeier, Marc
中科院分区：
文献类型：
--
作者：
Spisak, Shaun;Ostermeier, Marc
在合成生物学社区中，对调节基因表达的新方法存在持续的需求。蛋白质开关可以感知生物输入并以功能输出做出反应，代表了满足这一需求的一种方式。尽管事实上已经有大量的转录因子和信号蛋白可用，但现有的开关库缺乏某些应用所需的底物特异性和活性。因此，大量的技术已被应用于工程师的开关与新的属性。在这里，我们通过将它们大致组织成三种方法来讨论其中的一些技术。我们展示了如何通过诱变、结构域交换或结构域插入来创建新的开关。然后，我们简要地讨论了它们作为生物传感器和复杂的遗传电路中的用途。
There is an ongoing need in the synthetic biology community for novel ways to regulate gene expression. Protein switches, which sense biological inputs and respond with functional outputs, represent one way to meet this need. Despite the fact that there is already a large pool of transcription factors and signaling proteins available, the pool of existing switches lacks the substrate specificities and activities required for certain applications. Therefore, a large number of techniques have been applied to engineer switches with novel properties. Here we discuss some of these techniques by broadly organizing them into three approaches. We show how novel switches can be created through mutagenesis, domain swapping, or domain insertion. We then briefly discuss their use as biosensors and in complex genetic circuits.