Simple Spectroscopic Determination of Nitrate, Nitrite, and Ammonium in Arabidopsis thaliana
Simple Spectroscopic Determination of Nitrate, Nitrite, and Ammonium in Arabidopsis thaliana
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DOI：
10.21769/bioprotoc.2280
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发表时间：
2017-05-20
期刊：
影响因子：
0.8
通讯作者：
Okamoto, Yuki
中科院分区：
文献类型：
--
作者：
Hachiya, Takushi;Okamoto, Yuki
植物使用硝酸盐、亚硝酸盐和铵作为无机氮 (N) 源。这些氮化合物以不同的浓度包含在植物组织中，具体取决于它们的吸收和同化之间的平衡。因此，硝酸盐、亚硝酸盐和铵的含量是植物氮经济的生理指标。在这里，我们描述了用于测量拟南芥芽或根中这些无机氮物种的协议。
Plants use nitrate, nitrite, and ammonium as inorganic nitrogen (N) sources. These N compounds are included in plant tissues at various concentrations depending on the balance between their uptake and assimilation. Thus, the contents of nitrate, nitrite, and ammonium are physiological indicators of plant N economy. Here, we describe a protocol for measurement of these inorganic N species in A. thaliana shoots or roots.