A gonadotropin-releasing hormone (GnRH) receptor specific for GnRH II in primates.
A gonadotropin-releasing hormone (GnRH) receptor specific for GnRH II in primates.
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DOI：
10.1006/bbrc.2001.4678
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发表时间：
2001-04
影响因子：
3.1
通讯作者：
Jimmy D. Neill;Duck Lw;J. Sellers;L. Musgrove
中科院分区：
文献类型：
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作者：
Jimmy D. Neill;Duck Lw;J. Sellers;L. Musgrove
哺乳动物促性腺激素释放激素（GnRH I）是下丘脑十肽，与垂体细胞上的膜受体（I型）相互作用后刺激促性腺激素分泌，从而控制生殖过程。哺乳动物中表达的第二种释放激素（GnRH II）在早期被证明在非哺乳动物中表达，并有自己的受体。在这里，我们证明了第二个受体（II型）基因存在于人类基因组中，并报告其cDNA从猴子的克隆和表征。该cDNA编码具有C-末端胞质尾的G-蛋白偶联/7跨膜受体;它与两栖动物和鱼类的II型受体（约55%同一性）比与人类的I型受体（约39%）更相似。实验证明GnRH II型受体对GnRH II具有功能性和特异性。GnRH受体II型RNA在人体组织中广泛表达。这是哺乳动物中GnRH II型受体的首次报道。其鉴定将允许探索其在调节促性腺激素分泌、女性性行为和肿瘤细胞生长中的作用。
Mammalian gonadotropin-releasing hormone (GnRH I) is a hypothalamic decapeptide that stimulates gonadotropic hormone secretion upon interaction with its membrane receptors (type I) on pituitary cells, thereby governing reproductive processes. A second releasing hormone (GnRH II) expressed in mammals was shown earlier to be expressed in nonmammals and to have its own receptor. Here we demonstrate that a second receptor (type II) gene is present in the human genome, and report the cloning and characterization of its cDNA from monkeys. The cDNA encodes a G-protein-coupled/7 transmembrane receptor having a C-terminal cytoplasmic tail; it resembles more closely the type II receptors of amphibians and fish (approximately 55% identity) than it does the type I receptor of humans (approximately 39%). The GnRH type II receptor proved to be experimentally functional with, and specific for, GnRH II. GnRH receptor type II RNA is expressed ubiquitously in human tissues. This is the first report of a GnRH type II receptor in mammals. Its identification will permit exploration of its role in regulating gonadotropin secretion, female sexual behavior, and tumor cell growth.