Calcium-Phospholipid-Phosphate Complexes in Mineralizing Tissue 1
Calcium-Phospholipid-Phosphate Complexes in Mineralizing Tissue 1
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矿化组织中的钙-磷脂-磷酸盐复合物 1
DOI：
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发表时间：
1978
期刊：
影响因子：
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通讯作者：
A. S. Posner
中科院分区：
文献类型：
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作者：
A. Boskey;Mark R. Goldberg;A. S. Posner
概述已经使用先前描述的超声依赖性方法和新方法从矿化组织中分离出钙-磷脂-磷酸盐复合物，所述新方法依赖于使用酶从在温和条件下脱钙的组织中除去非脂质基质。两种方法的结果相当。复合物被发现是所有矿化的兔子组织的成分;骨，钙化软骨和牙齿。没有这样的复合物可以从非矿化组织分离;肌肉，耳软骨，骨髓，脂肪组织。Ca-PL-PO 4的比例在参与活跃矿化的那些组织中最高，即，年轻的组织。从矿化组织中分离的所有复合物含有50摩尔%的Ca，以及可变量的酸性磷脂和无机磷酸盐。然而，钙：总磷的摩尔比总是统一的。所有分离的复合物显示诱导在体外从亚稳磷酸钙溶液形成羟基磷灰石，因此，被认为是与体内钙化。
Summary Calcium - phospholipid - phosphate complexes have been isolated from mineralized tissues using a sonication-dependent method described previously and a new method which depends on the use of enzymes to remove the nonlipid matrix from tissues which were decalcified under mild conditions. Both methods gave comparable results. The complexes were found to be constituents of all mineralized rabbit tissues; bone, calcified cartilage, and tooth. No such complexes could be isolated from the nonmineralized tissues; muscle, ear cartilage, bone marrow, adipose tissue. The proportion of Ca-PL-PO4 is highest in those tissues which are involved in active mineralization, i.e., the younger tissues. All complexes isolated from mineralized tissues contain 50 mole % Ca, and variable amounts of the acidic phospholipids and inorganic phosphate. However, the Ca:total P molar ratio was always unity. All isolated complexes were shown to induce in vitro hydroxyapatite formation from metastable calcium phosphate solution and, thus, are believed to be associated with in vivo calcification.