Measurement of long-chain fatty acid uptake into adipocytes.
Measurement of long-chain fatty acid uptake into adipocytes.
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DOI：
10.1016/b978-0-12-800280-3.00007-4
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发表时间：
2014
影响因子：
--
通讯作者：
Stahl, Andreas
中科院分区：
文献类型：
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作者：
Dubikovskaya, Elena;Chudnovskiy, Rostislav;Karateev, Grigory;Park, Hyo Min;Stahl, Andreas
白色和棕色脂肪组织有效摄取长链脂肪酸的能力是它们分别在能量储存和产热方面的生理功能的关键。已经采取了几种方法来确定培养细胞以及原代脂肪细胞的摄取率，包括放射性和荧光标记的脂肪酸。此外，最近的可激活的生物发光脂肪酸的描述已经打开了扩展这些体外方法的可能性，以实时监测体内脂肪库的脂肪酸摄取动力学。在这里，我们将描述一些最有用的实验范例，以定量测定长链脂肪酸摄取的脂肪细胞在体外，并提供详细的说明，如何在体内成像的生物发光探针可以合成和使用在活体小鼠的读者。
The ability of white and brown adipose tissue to efficiently take up long-chain fatty acids is key to their physiological functions in energy storage and thermogenesis respectively. Several approaches have been taken to determine uptake rates by cultured cells as well as primary adipocytes including radio- and fluorescently labeled fatty acids. In addition, the recent description of activatable bioluminescent fatty acids has opened the possibility for expanding these in vitro approaches to real-time monitoring of fatty acid uptake kinetics by adipose depots in vivo. Here we will describe some of the most useful experimental paradigms to quantitatively determine long-chain fatty acid uptake by adipocytes in vitro and provide the reader with detailed instruction on how bioluminescent probes for in vivo imaging can be synthesized and used in living mice.