Expression of three immunoglobulin isotypes by individual B cells during development: implications for heavy chain switching.
Expression of three immunoglobulin isotypes by individual B cells during development: implications for heavy chain switching.
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发育过程中单个 B 细胞表达三种免疫球蛋白同种型：对重链转换的影响。
DOI：
10.1111/j.1600-0897.1981.tb00028.x
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Lawton,AR
中科院分区：
文献类型：
--
作者：
Gandini,M;Kubagawa,H;Gathings,WE;Lawton,AR
先前对小鼠和人类的研究表明，在个体发育过程中出现的第一批IgA+和IgG+B细胞表面也携带IgM。IgD的获得似乎发生在分化的较晚阶段。在这项研究中，我们将放射自显影与双色免疫荧光相结合，直接检测表达三种表面同种型的人B细胞。我们报道，新生儿中绝大多数IgA+细胞也携带IgM和IgD；IgG+细胞也是如此。这种表型是新生儿特有的，而在成人中，大多数IgA+和IgG+细胞是单一的。我们讨论了这一发现对重链转换的遗传意义。
Previous studies in mice and humans have shown that the first IgA+and IgG+B cells appearing during ontogeny also bear IgM on the surface. The acquisition of IgD seems to occur at a later stage in differentiation. In this study we have combined autoradiography with two‐color immunofluorescence to directly detect human B cells expressing three surface isotypes. We report that the vast majority of IgA+cells in the neonate also bear IgM and IgD; the same holds true for IgG+cells. This phenotype is peculiar of newborns, while in the adult the majority of IgA+and IgG+cells are single. We discuss the genetic implications of such a finding for heavy chain switching.