Detection of quantitative trait loci influencing somatic cell score in Spanish Churra sheep
Detection of quantitative trait loci influencing somatic cell score in Spanish Churra sheep
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DOI：
10.3168/jds.s0022-0302(07)72643-7
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发表时间：
2007-01-01
影响因子：
3.5
通讯作者：
Arranz, J. J.
中科院分区：
文献类型：
--
作者：
Gutierrez-Gil, B.;El-Zarei, M. F.;Arranz, J. J.
11个半同胞绵羊家族，包括1421只西班牙丘拉绵羊，被分析了覆盖整个常染色体绵羊基因组的181个微卫星标记。采用多标记回归方法，采用子代试验设计确定影响体细胞评分的数量性状基因座(QTL)。染色体意义阈值是通过置换表型数据来设定的。为该商品群构建的常染色体连锁图谱的标记顺序和遗传距离与已发表的绵羊连锁图谱一致。跨家族关联分析显示，20号染色体上有一个区域暗示存在QTL的证据。从家系内分析推断，QTL被分离到2个家系中，所建议的QTL在两个半同胞群体中的位置存在差异。这可能是与重要家族的标记相关的信息不完整的结果。主要组织相容性复合体的位置接近家族交叉效应，这表明该区域可能包含一个分离的QTL，用于丘拉种群的SCS。在奶牛中检测SCS的研究报告了与相应的牛直源区域的连锁关系，支持了我们的发现的有效性。
Eleven half-sib ovine families, including 1,421 Spanish Churra ewes, were analyzed for 181 microsatellite markers spanning the entire autosomic ovine genome. Using a multimarker regression method, a daughter experimental design was used to identify putative quantitative trait loci (QTL) affecting the somatic cell score (SCS). Chromosome-wise significance thresholds were set empirically by permuting the phenotypic data. Marker order and genetic distances of the autosomic linkage map built for this commercial population were in accordance with the published ovine linkage map. An across-family association analysis revealed a region on chromosome 20 suggestive of evidence for a QTL. Segregation of the QTL into 2 families was inferred from the within-family analysis, and differences in the position of the suggested QTL were found between the 2 half-sib groups. This could be the result of incomplete information associated with the markers for the significant families. The location of the major histocompatibility complex in proximity to the across-family effect suggests this region may harbor a segregating QTL for the SCS in the Churra population. Studies in dairy cattle examining the SCS have reported linkage associations on corresponding bovine orthologous regions, supporting the validity of our findings.