RECOGNITION OF HISTOPLASMA-CAPSULATUM YEAST-CELL ANTIGENS BY HUMAN-LYMPHOCYTES AND HUMAN T-CELL CLONES
RECOGNITION OF HISTOPLASMA-CAPSULATUM YEAST-CELL ANTIGENS BY HUMAN-LYMPHOCYTES AND HUMAN T-CELL CLONES
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DOI：
10.1002/jlb.51.5.432
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发表时间：
1992-05-01
影响因子：
5.5
通讯作者：
DEEPE, GS
中科院分区：
文献类型：
--
作者：
HENDERSON, HM;DEEPE, GS
我们已经从荚膜组织胞浆菌酵母细胞的细胞壁和细胞膜制备了洗涤剂提取物，其被来自用活生物体或提取物免疫的小鼠的T细胞识别。已从该提取物中分离出一种62 kd抗原，并已证明其具有抗原性，可在小鼠中赋予保护性免疫力。在这项研究中，我们研究了人淋巴细胞和人T细胞克隆的细胞壁和细胞膜的提取物和62 kd抗原，称为HIS-62的体外增殖反应。7名健康人的外周血单个核细胞对H.荚膜酵母细胞来自所有7个个体的单核细胞识别组织胞浆菌。使用细胞壁和细胞膜的提取物作为抗原制剂，从单个人的外周血单核细胞产生T细胞克隆。繁殖了19个克隆;所有克隆均表达表面表型CD 3+、CD 4+、T细胞受体α/β +。研究的17个克隆，没有反应的提取物从芽生孢子菌dermativitis或粗球孢子菌或破伤风类毒素。19个克隆中有16个在体外识别HIS-62。进一步的分析显示，对提取物有反应的七个人中的每一个人的细胞都对HIS-62产生了增殖反应。因此，来自细胞壁和细胞膜的提取物和HIS-62是人细胞介导的对H.荚膜此外，HIS-62似乎是免疫显性抗原。
We have prepared a detergent extract from the cell wall and cell membrane of Histoplasma capsulatum yeast cells that is recognized by T cells from mice immunized with viable organisms or with the extract. A 62-kd antigen from this extract has been isolated and has been shown to be antigenic and to confer protective immunity in mice. In this study, we examined the in vitro proliferative response by human lymphocytes and human T-cell clones to both the extract from the cell wall and cell membrane and the 62-kd antigen, termed HIS-62. Seven healthy individuals were identified whose peripheral blood mononuclear cells responded to the cell wall and cell membrane extract from H. capsulatum yeast cells. Mononuclear cells from all seven individuals recognized histoplasmin. T-cell clones were generated from peripheral blood mononuclear cells from a single person using the extract from cell wall and cell membrane as the antigenic preparation. Nineteen clones were propagated; all expressed the surface phenotype CD3+, CD4+, T-cell receptor alpha/beta+ The clones were antigen-specific. Of 17 clones studied, none responded to extracts from Blastomyces dermatitidis or Coccidioides immitis or to tetanus toxoid. Sixteen of 19 clones recognized HIS-62 in vitro. Additional analysis revealed that cells from each of the seven individuals who responded to the extract mounted a proliferative response to HIS-62. Thus, the extract from the cell wall and cell membrane and HIS-62 are targets of the human cell-mediated immune response to H. capsulatum. Moreover, HIS-62 appears to be an immunodominant antigen.