The acidocalcisome vacuolar transporter chaperone 4 catalyzes the synthesis of polyphosphate in insect-stages of Trypanosoma brucei and T. cruzi.
The acidocalcisome vacuolar transporter chaperone 4 catalyzes the synthesis of polyphosphate in insect-stages of Trypanosoma brucei and T. cruzi.
复制标题
酸钙体液泡转运蛋白伴侣 4 在布氏锥虫和克氏锥虫昆虫阶段催化多磷酸盐的合成。
DOI：
10.1111/jeu.12093
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Docampo,Roberto
中科院分区：
文献类型：
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作者：
Ulrich,PaulN;Lander,Noelia;Kurup,SamarchithP;Reiss,Laura;Brewer,Jessica;SoaresMedeiros,LiaC;Miranda,Kildare;Docampo,Roberto
Polyphosphate is a polymer of inorganic phosphate found in both prokaryotes and eukaryotes. Polyphosphate typically accumulates in acidic, calcium‐rich organelles known as acidocalcisomes, and recent research demonstrated that vacuolar transporter chaperone 4 catalyzes its synthesis in yeast. The human pathogensTrypanosoma bruceiandT. cruzipossess vacuolar transporter chaperone 4 homologs. We demonstrate thatT. cruzivacuolar transporter chaperone 4 localizes to acidocalcisomes of epimastigotes by immunofluorescence and immuno‐electron microscopy and that the recombinant catalytic region of theT. cruzienzyme is a polyphosphate kinase. RNA interference of theT. bruceienzyme in procyclic form parasites reduced short chain polyphosphate levels and resulted in accumulation of pyrophosphate. These results suggest that this trypanosome enzyme is an important component of a polyphosphate synthase complex that utilizes ATP to synthesize and translocate polyphosphate to acidocalcisomes in insect stages of these parasites.
