Tissue shrinkage and unbiased stereological estimation of particle number and size
Tissue shrinkage and unbiased stereological estimation of particle number and size
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DOI：
10.1046/j.1365-2818.2001.00958.x
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发表时间：
2001-12-01
期刊：
影响因子：
--
通讯作者：
Gundersen, HJG
中科院分区：
文献类型：
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作者：
Dorph-Petersen, KA;Nyengaard, JR;Gundersen, HJG
本文综述了在存在组织变形时与粒子数目和尺寸的无偏估计有关的体视学问题。变形可以在组织的组织学处理期间发生。特别要注意的是，广泛使用的光学剥离器可以通过z轴（即垂直于截面的方向）上的尺寸变化而偏置。当冷冻切片或振动切片机切片用于体视学测量时，情况往往如此。本文介绍了新的估计将用于光学分馏器和光学disector设计：第一个是，像往常一样，最简单和最强大的。最后，它是说，当组织变形只发生在z方向，无偏估计的颗粒大小与几个估计是可能的。
This paper is a review of the stereological problems related to the unbiased estimation of particle number and size when tissue deformation is present. The deformation may occur during the histological processing of the tissue. It is especially noted that the widely used optical disector may be biased by dimensional changes in the z-axis, i.e. the direction perpendicular to the section plane. This is often the case when frozen sections or vibratome sections are used for the stereological measurements. The present paper introduces new estimators to be used in optical fractionator and optical disector designs: the first is, as usual, the simplest and most robust. Finally, it is stated that when tissue deformation only occurs in the z-direction, unbiased estimation of particle size with several estimators is possible.