A facile fluorescent sensor based on carbon dots and Fe3O4 nanoplates for the detection of hyaluronidase activity
A facile fluorescent sensor based on carbon dots and Fe3O4 nanoplates for the detection of hyaluronidase activity
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DOI：
10.1016/j.snb.2021.130434
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发表时间：
2021-11
影响因子：
8.4
通讯作者：
Qiutong Chen;Sihan Rong;Yao Cen;Guanhong Xu;Z. Xie;Jing Yang;Yong Sun;Qin Hu;Fangdi Wei-
中科院分区：
文献类型：
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作者：
Qiutong Chen;Sihan Rong;Yao Cen;Guanhong Xu;Z. Xie;Jing Yang;Yong Sun;Qin Hu;Fangdi Wei-
由于单独使用碳量子点（CD）的传感平台的灵敏度很差，因此基于CD与其他纳米材料组合的传感器的开发引起了相当大的兴趣。本文建立了一种新的、简便的利用环糊精（CD）和Fe 3 O 4纳米片（NPs）测定透明质酸酶（HAase）活性的方法。首次引入Fe 3 O 4纳米粒子通过内滤效应（IFE）猝灭CD的荧光。带负电荷的HA可以通过静电吸引与带正电荷的Fe 3 O 4 NPs结合，形成HA/Fe 3 O 4 NPs复合物，从而削弱Fe 3 O 4 NPs与CD之间的IFE。因此，CD的荧光被恢复。在HAase的存在下，HA被水解成小片段，这些小片段不能与Fe 3 O 4 NPs相互作用。因此，CD的荧光再次被Fe 3 O 4 NPs“关闭”。HAase的活性测定范围为0.0040 - 0.12 U mL-1，检测限为0.0016 U mL-1。CD和Fe 3 O 4 NPs都是无标记的，低成本和生态友好的，使得检测系统简单且经济。该方法为新型传感平台的设计提供了一种新的思路。
Since the sensitivity of the sensing platform using carbon dots (CDs) alone is poor, the development of sensors based on the combination of CDs with other nanomaterials has attracted considerable interest. In this paper, a novel and facile method was established using CDs and Fe3O4nanoplates (NPs) for the determination of hyaluronidase (HAase) activity. Fe3O4NPs were introduced to quench the fluorescence of CDs through inner filter effect (IFE) for the first time. The negatively charged HA could bind to the positively charged Fe3O4NPs via electrostatic attraction to form HA/Fe3O4NPs complex, resulting in the weakening of the IFE between Fe3O4NPs and CDs. Hence, the fluorescence of CDs was recovered. With the presence of HAase, HA was hydrolyzed into small fragments which were unable to interact with Fe3O4NPs. Thus, the fluorescence of CDs was “turn-off” again by Fe3O4NPs. The activity of HAase was determined in a range of 0.0040−0.12 U mL−1with a limit of detection of 0.0016 U mL−1. Both the CDs and Fe3O4NPs were label-free, low-cost and eco-friendly, making the detection system simple and economical. The proposed method provides a new way of designing new sensing platform.