The spectrum of DNA damage in human sperm assessed by single cell gel electrophoresis (Comet assay) and its relationship to fertilization and embryo development
The spectrum of DNA damage in human sperm assessed by single cell gel electrophoresis (Comet assay) and its relationship to fertilization and embryo development
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DOI：
10.1093/humrep/17.4.990
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发表时间：
2002-04-01
影响因子：
6.1
通讯作者：
Brison, DR
中科院分区：
文献类型：
--
作者：
Morris, ID;Ilott, S;Brison, DR
背景资料：精子DNA的完整性对于自然或辅助受精的成功以及胚胎、胎儿和儿童的正常发育至关重要。ICSI通过绕过精子选择机制，增加了传递受损DNA的风险，其重要性需要调查。研究方法：通过单细胞凝胶电泳（彗星试验）测定了60名接受IVF治疗的男性中精子的DNA损伤，并与精液和治疗周期参数相关。结果：精子DNA损伤的范围广泛，在男性内和男性之间都有，但没有确定特定的亚组。精液和治疗周期参数在根据精子DNA损伤程度高低分组的男性中没有差异。但回归分析显示，DNA损伤与年龄（29-44岁）、精子异常和活动力呈正相关，与精子浓度呈负相关。在ICSI周期中，DNA损伤与受精后胚胎卵裂受损呈正相关。结论：本研究为精子DNA损伤提供了证据。彗星试验测得的高负荷DNA损伤可预测ICSI后胚胎发育失败。由于DNA损伤的精子可能有助于成功受精和体外发育，因此潜在的不良影响仍有待澄清。
Background: The integrity of sperm DNA is important for the success of natural or assisted fertilization, as well as normal development of the embryo, fetus and child. ICSI, by bypassing sperm selection mechanisms, increases the risk of transmitting damaged DNA and the significance of this requires investigation. Methods: DNA damage in sperm from an unselected group of 60 men undergoing IVF treatment was measured by single cell gel electrophoresis (Comet assay) and correlated with semen and treatment cycle parameters. Results: Wide spectra of sperm DNA damage were found both within and between men but no specific subgroups were identified. Semen and treatment cycle parameters were not different in men grouped according to high or low sperm DNA damage. However, regression analysis showed that DNA damage was positively associated with age (29-44 years), abnormal sperm and motility and negatively associated with sperm concentration. In ICSI cycles DNA damage was positively associated with impairment of post-fertilization embryo cleavage. Conclusions: This study contributes to the evidence of DNA damage within sperm. High loads of DNA damage measured by the Comet assay were predictive of failure of embryo development after ICSI. As it is likely that sperm with DNA damage contributed to successful fertilization and in-vitro development, potential adverse effects remain to be clarified.