Polymerase chain reaction diagnosis of primary human herpesvirus-6 infection in the acute care setting.
Polymerase chain reaction diagnosis of primary human herpesvirus-6 infection in the acute care setting.
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急性护理环境中原发性人类疱疹病毒 6 感染的聚合酶链反应诊断。
DOI：
10.1016/j.jpeds.2006.05.027
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Corey,Lawrence
中科院分区：
文献类型：
--
作者：
Zerr,DanielleM;Frenkel,LisaM;Huang,Meei-Li;Rhoads,Margaret;Nguy,Long;DelBeccaro,MarkA;Corey,Lawrence
OBJECTIVETo evaluate the potential utility of identifying primary human herpesvirus (HHV)-6 infection in an emergency department setting by determining the frequency of HHV-6 viremia, diagnostic testing, and empiric treatment of serious bacterial infection (SBI) in HHV-6 viremic children, and concurrent SBI and HHV-6 viremia.STUDY DESIGNChildren under age 2 years and who had a blood specimen taken for evaluation of fever were tested for HHV-6 by polymerase chain reaction (PCR). HHV-6 viremia was defined as detection of HHV-6 DNA in acute plasma.RESULTSA total of 32 of the 181 subjects (18%) had HHV-6 viremia. Children with HHV-6 viremia frequently underwent procedures for diagnosis and empiric treatment of SBI: 60% had bladder catheterizations, 6% had lumbar punctures, 47% had radiographs, 32% received empiric antibiotics, and 34% were hospitalized. Four of the 32 children with HHV-6 viremia (12.5%) were diagnosed with SBI, although none had a positive culture of blood or cerebrospinal fluid.CONCLUSIONSRapid diagnosis of HHV-6 viremia may not serve to adequately differentiate infants with and without SBI in acute care settings. Although no children with HHV-6 viremia had bacteremia or meningitis, it appears that additional criteria are needed to increase the specificity of HHV-6 PCR testing before withholding evaluation for SBI.
