The ORGAN SIZE (ORG) locus modulates both vegetative and reproductive gigantism in domesticated tomato.
The ORGAN SIZE (ORG) locus modulates both vegetative and reproductive gigantism in domesticated tomato.
复制标题
器官大小 (ORG) 基因座调节驯化番茄的营养和生殖巨人症。
DOI：
10.1093/aob/mcad150
复制
发表时间：
2023
期刊：
影响因子：
4.2
通讯作者：
Vicente MH
中科院分区：
文献类型：
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作者：
Vicente MH
背景和目的巨人是驯化综合症的关键组成部分，驯化综合症是区分作物与野生近缘作物的一系列特征。异速生长巨人症在园艺作物中非常明显，导致茎、叶或果实等可食部分的大小不成比例地增加。番茄(Solanum Lycopersicum)作为果实巨大症的模型引起了人们的关注，已有许多基因被描述控制这一性状。然而，营养器官的大小相应增加导致等长巨体的遗传基础还没有被探索。方法在两个不同的番茄遗传背景(cv.)中，我们在7号染色体上的一个0.4-Mb区域定位于野生种茄子的导入系(ILs)。‘M82’和cv.控制番茄营养器官和生殖器官大小的基因。该基因座被命名为ORGAN SIZE(ORG)，并使用逐个测序的基因型进行了精细定位。研究表明，在驯化过程中，控制番茄果实重量的数量性状基因座发生重叠。关键结果来自野生种的等位基因导致番茄不同器官中的细胞数量减少，这在一定程度上被叶片中较大的细胞膨大所补偿，但在果实中没有。结果是携带野生种等位基因的自交系叶片、花朵和果实的大小成比例地减小。结论我们的发现表明，驯化过程中对大果实的选择也倾向于通过影响细胞分裂来选择叶片大小的增加。由于叶片大小与源库平衡和作物对不同环境的适应有关，ORG的发现可能允许对这些参数进行微调。
Background and AimsGigantism is a key component of the domestication syndrome, a suite of traits that differentiates crops from their wild relatives. Allometric gigantism is strongly marked in horticultural crops, causing disproportionate increases in the size of edible parts such as stems, leaves or fruits. Tomato (Solanum lycopersicum) has attracted attention as a model for fruit gigantism, and many genes have been described controlling this trait. However, the genetic basis of a corresponding increase in size of vegetative organs contributing to isometric gigantism has remained relatively unexplored.MethodsHere, we identified a 0.4-Mb region on chromosome 7 in introgression lines (ILs) from the wild speciesSolanum pennelliiin two different tomato genetic backgrounds (cv. ‘M82’ and cv. ‘Micro-Tom’) that controls vegetative and reproductive organ size in tomato. The locus, namedORGAN SIZE(ORG), was fine-mapped using genotype-by-sequencing. A survey of the literature revealed thatORGoverlaps with previously mapped quantitative trait loci controlling tomato fruit weight during domestication.Key ResultsAlleles from the wild species led to lower cell number in different organs, which was partially compensated by greater cell expansion in leaves, but not in fruits. The result was a proportional reduction in leaf, flower and fruit size in the ILs harbouring the alleles from the wild species.ConclusionsOur findings suggest that selection for large fruit during domestication also tends to select for increases in leaf size by influencing cell division. Since leaf size is relevant for both source–sink balance and crop adaptation to different environments, the discovery ofORGcould allow fine-tuning of these parameters.