Cross species multi‐omics reveals cell wall sequestration and elevated global transcript abundance as mechanisms of boron tolerance in plants
Cross species multi‐omics reveals cell wall sequestration and elevated global transcript abundance as mechanisms of boron tolerance in plants
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跨物种多组学揭示细胞壁隔离和整体转录丰度升高是植物耐硼的机制
DOI：
10.1111/nph.17295
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发表时间：
2021
期刊：
影响因子：
9.4
通讯作者：
Dassanayake, Maheshi
中科院分区：
文献类型：
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作者：
Wang, Guannan;DiTusa, Sandra Feuer;Oh, Dong‐Ha;Herrmann, Achim D.;Mendoza‐Cozatl, David G.;O'Neill, Malcolm A.;Smith, Aaron P.;Dassanayake, Maheshi
硼毒害是一个世界性的问题，但我们对植物对这种胁迫的遗传反应和适应机制的了解有限。我们采用了硼胁迫敏感的拟南芥（Arabidopsis thaliana）和其耐硼胁迫的极端植物近缘种小施伦凯氏菌（Schrenkiella parvula）的跨物种比较，并采用了整合基因组学、转录组学、研究结果表明，在过量硼胁迫下，小施氏菌（Schrenkiella parvulamaB.）的总硼和游离硼浓度低于拟南芥，而小施氏菌（Schrenkiella parvulamaB.）硼更有效地比拟南芥，我们建议部分驱动SpBOR5，硼转运蛋白，我们在这项研究中的功能特点。这两个物种使用细胞壁作为过量硼的部分汇。当在细胞质中积累时，过量的硼似乎会中断RNA代谢。极端植物。在维持可与硼酸结合的含核糖化合物库的同时，小链球菌促进关键的细胞过程。小链球菌转录组预先适应硼毒性。它表现出与拟南芥硼胁迫响应转录组的大量重叠。过量硼条件下，细胞壁螯合和全球转录水平的增加成为维持硼毒性下植物生长的关键机制。
Boron toxicity is a world‐wide problem for crops, yet we have a limited understanding of the genetic responses and adaptive mechanisms to this stress in plants.We employed a cross‐species comparison between boron stress‐sensitiveArabidopsis thalianaand its boron stress‐tolerant extremophyte relativeSchrenkiella parvula, and a multi‐omics approach integrating genomics, transcriptomics, metabolomics and ionomics to assess plant responses and adaptations to boron stress.Schrenkiella parvulamaintains lower concentrations of total boron and free boric acid than Arabidopsis when grown with excess boron.Schrenkiella parvulaexcludes excess boron more efficiently than Arabidopsis, which we propose is partly driven by SpBOR5, a boron transporter that we functionally characterize in this study. Both species use cell walls as a partial sink for excess boron. When accumulated in the cytoplasm, excess boron appears to interrupt RNA metabolism. The extremophyteS. parvulafacilitates critical cellular processes while maintaining the pool of ribose‐containing compounds that can bind with boric acid.TheS. parvulatranscriptome is pre‐adapted to boron toxicity. It exhibits substantial overlaps with the Arabidopsis boron‐stress responsive transcriptome. Cell wall sequestration and increases in global transcript levels under excess boron conditions emerge as key mechanisms for sustaining plant growth under boron toxicity.