THYROID-HORMONE ATTENUATES AND AUGMENTS HEPATIC GENE-EXPRESSION AT A PRE-TRANSLATIONAL LEVEL
THYROID-HORMONE ATTENUATES AND AUGMENTS HEPATIC GENE-EXPRESSION AT A PRE-TRANSLATIONAL LEVEL
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DOI：
10.1073/pnas.78.8.4733
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
OPPENHEIMER, JH
中科院分区：
文献类型：
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作者：
SEELIG, S;LIAW, C;OPPENHEIMER, JH
在甲状腺功能减退和甲状腺功能正常之间以及甲状腺功能正常和甲状腺功能亢进之间的过渡中，确定了大鼠肝脏基因组受甲状腺激素选择性影响的比例，并描述了个体mRNA序列的反应模式。从甲状腺切除、完整、甲状腺功能正常大鼠和甲状腺切除大鼠的肝脏中提取Poly（A）+RNA，使甲状腺功能正常和甲状腺功能亢进与每日剂量的三碘甲腺原氨酸。在[35S]蛋氨酸存在下，将提取的RNA在网织红细胞裂解物系统中翻译，并通过二维凝胶电泳分析产物。三碘甲状腺原氨酸在翻译前水平上减弱和增强某些基因的表达。这可能代表激素的直接或间接作用。三碘甲腺原氨酸影响约8%的231个mRNA序列可视化，刺激活性在11和抑制活性在7个序列。与甲状腺功能正常的动物相比，甲状腺切除和甲状腺功能亢进的动物中至少有1个mRNA序列的翻译活性降低。mRNA响应与受体占有率的关系随线性和扩增响应以及在小于完全核占有率时最大的响应的实例而变化。
The proportion of the rat hepatic genome that is under the selective influence of thyroid hormones was ascertained and the response patterns of individual mRNA sequences were described in the transition between hypothyroidism and euthyroidism and between euthyroidism and hyperthyroidism. Poly(A)+RNA was extracted from livers of thyroidectomized, intact, euthyroid rats and of thyroidectomized rats rendered euthyroid and hyperthyroid with daily doses of triiodothyronine. The extracted RNA was translated in a reticulocyte lysate system in the presence of [35S]methionine, and the products were analyzed by 2-dimensional gel electrophoresis. Triiodothyronine attenuates as well as augments the expression of certain genes at a pretranslational level. This could represent either a direct or an indirect action of the hormone. Triiodothyronine influences approximately 8% of the 231 mRNA sequences visualized, stimulating activity in 11 and inhibiting activity in 7 sequences. Translational activity of at least 1 mRNA sequence decreased in both thyroidectomized and hyperthyroid animals, compared to euthyroid levels. The relationship of mRNA response to receptor occupancy varied with examples of linear and amplified responses and responses that were maximal at less than full nuclear occupancy.