A MUTATION DATA MATRIX FOR TRANSMEMBRANE PROTEINS
A MUTATION DATA MATRIX FOR TRANSMEMBRANE PROTEINS
复制标题
DOI：
10.1016/0014-5793(94)80429-x
复制
发表时间：
1994-02-21
期刊：
影响因子：
3.5
通讯作者：
THORNTON, JM
中科院分区：
文献类型：
--
作者：
JONES, DT;TAYLOR, WR;THORNTON, JM
广泛使用的突变数据矩阵（MDM）是一种氨基酸比较矩阵，通过对交换概率（或几率）的研究计算得出，该交换概率（或几率）来自对非常相似的蛋白质组中观察到的进化变化的分析。在这项工作中，突变数据矩阵计算跨膜段。这个新的突变数据矩阵被发现是非常不同的矩阵计算从一般序列集偏向于水溶性球状蛋白质，和差异的背景下，跨膜段的特定结构要求进行了讨论。这种新的矩阵将有助于提高完整膜蛋白序列比对的准确性，也可以用于这类蛋白质定点突变实验的合理设计
The widely used Mutation Data Matrix (MDM), is an amino acid comparison matrix calculated from a study of the exchange probabilities (or odds) derived from an analysis of the evolutionary changes seen in groups of very similar proteins. In this work, a mutation data matrix is calculated for membrane spanning segments. This new mutation data matrix is found to be very different from matrices calculated from general sequence sets which are biased towards water-soluble globular proteins, and the differences are discussed in the context of specific structural requirements of membrane spanning segments. This new matrix will help improve the accuracy of integral membrane protein sequence alignments, and could also be of use in the rational design of site directed mutagenesis experiments for this class of proteins