lsolation and Characterization of Three 14-Kilodalton Proteins of Tomato and of a Basic PR-1 of Tobacco with lnhibitory Activity against Phyfophfhora infestans

lsolation and Characterization of Three 14-Kilodalton Proteins of Tomato and of a Basic PR-1 of Tobacco with lnhibitory Activity against Phyfophfhora infestans
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发表时间：
2002
期刊：
The Journal of molecular diagnostics : JMD
影响因子：
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通讯作者：
T. Niderman;lsabelle Genetet;Thierry Bruyère;A. Stintzi;M. Legrand
T. Niderman;lsabelle Genetet;Thierry Bruyère;A. Stintzi;M. Legrand
中科院分区：
其他
文献类型：
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作者：
T. Niderman;lsabelle Genetet;Thierry Bruyère;A. Stintzi;M. Legrand

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从番茄中分离到三种不同的碱性14-kD蛋白，即P14a、P14b和P14c。CV Baby)感染致病疫霉的叶片。它们在体外(抑制游动孢子萌发)和体内番茄叶盘试验(感染叶表面减少)中都表现出对致病疫霉的抗真菌活性。血清学交叉反应和氨基酸序列比较表明，这三种蛋白均属于PR-1致病相关蛋白。P14a和P14b与先前鉴定的P14有很高的相似性，而P14c与从分离的DNA克隆中预测的烟草基本类型PR-1非常相似。该蛋白质命名为PR-1g，是从感染病毒的烟草叶片中纯化的，并与已知的酸性烟草PR-1a、PR-1b和PR-1c一起进行了氨基酸显微测序。L比较了不同的番茄和烟草PR-1蛋白的生物活性，发现它们在体外和体内对致病疫霉具有不同的杀菌活性，其中最有效的是新鉴定的番茄P14c和烟草PR-1g。
Three distinct basic 14-kD proteins, P14a, P14b, and P14c, were isolated from tomato (Lycopersicon esculentum Mill. cv Baby) leaves infected with Phytophthora infestans. They exhibited antifungal activity against P. infestam both in vitro (inhibition of zoospore germination) and in vivo with a tomato leaf disc assay (decrease in infected leaf surface). Serological cross-reactions and amino acid sequence comparisons showed that the three proteins are members of the PR-1 group of pathogenesis-related (PR) proteins. P14a and P14b showed high similarity to a previously characterized P14, whereas P14c was found to be very similar to a putative basic-type PR-1 from tobacco predicted from isolated DNA clones. This protein, named PR-1 g, was purified from virus-infected tobacco (Nicotiana tabacum Samsun NN) leaves and characterized by amino acid microsequencing, along with the well-known acidic tobacco PR-la, PR-lb, and PR-lc. l h e various tomato and tobacco PR-1 proteins were compared for their biological activity and found to display differential fungicidal activity against P. infestam in both the in vitro and in vivo assays, the most efficient being the newly characterized tomato P14c and tobacco PR-1 g.