Purification and characterization of the diphtheria toxin repressor.
Purification and characterization of the diphtheria toxin repressor.
复制标题
白喉毒素阻遏物的纯化和表征。
DOI：
10.1073/pnas.89.16.7576
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发表时间：
1992
影响因子：
11.1
通讯作者：
Holmes,RK
中科院分区：
文献类型：
--
作者：
Schmitt,MP;Twiddy,EM;Holmes,RK
白喉毒素抑制基因（dtxR）编码一种蛋白（dtxR），该蛋白通过铁依赖机制调节白喉毒素基因（tox）的转录。利用噬菌体T7基因10启动子在大肠杆菌中表达克隆的dtxR，并纯化了dtxR。DtxR与tox+操作符的特异性结合依赖于DtxR的还原和铁离子的存在。DtxR保护了大约30对核苷酸序列，部分重叠tox启动子并包含一个双对称区域，不被dna酶i消化。DtxR与突变的tox-201操作区域结合很少，不能与大肠杆菌铁摄取调节（fur）基因的启动子/操作区域结合。
The diphtheria toxin repressor gene (dtxR) encodes a protein (DtxR) that regulates transcription of the diphtheria toxin gene (tox) by an iron-dependent mechanism. Cloned dtxR was expressed in Escherichia coli from the phage T7 gene 10 promoter, and DtxR was purified. Specific binding of DtxR to the tox+ operator was dependent on reduction of DtxR and the presence of ferrous ions. DtxR protected a sequence of approximately 30 nucleotide pairs, partially overlapping the tox promoter and containing a region of dyad symmetry, from digestion by DNase I. DtxR exhibited very little binding to the mutant tox-201 operator region and failed to bind to the promoter/operator region of the ferric uptake regulation (fur) gene of E. coli.
影响因子：
4.3
作者：
H. Sambrook
通讯作者：
H. Sambrook
温带噬菌体携带的白喉毒素结构基因突变。
DOI：
10.1038/newbio233008a0
发表时间：
1971
期刊：
Nature: New biology
影响因子：
--
作者：
T. Uchida;Gill Dm;A. M. Pappenheimer
通讯作者：
A. M. Pappenheimer