Short-hairpin RNA-mediated suppression of cortactin may inhibit the migration and invasion abilities of endometrial cancer cells by reducing lamellipodia.
Short-hairpin RNA-mediated suppression of cortactin may inhibit the migration and invasion abilities of endometrial cancer cells by reducing lamellipodia.
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DOI：
10.22038/ijbms.2023.67633.14863
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发表时间：
2023
影响因子：
2.2
通讯作者：
中科院分区：
文献类型：
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作者：
子宫内膜癌（EC）的预后受肿瘤浸润和转移的影响。Cordyn（CTTN）调节其他肿瘤的浸润和转移。CTTN在EC中的作用及其机制的研究有限，需要进一步的研究。采用定量PCR和免疫组织化学方法检测食管癌和正常组织中Ras相关C3肉毒毒素底物1（Rac 1）和CTTN的表达。分析这两个基因的表达与预后因素的关系。构建CTTN-RNAi慢病毒系统并转染EC细胞。通过划痕试验、transwell迁移试验和侵袭试验评估迁移和侵袭。免疫荧光染色观察伪足形成。Western blotting检测Rac 1的表达。Rac 1和CTTN在食管癌组织中的表达水平明显高于正常组织。在EC组中，Rac 1和CTTN水平相关。Rac 1和CTTN蛋白表达水平与子宫肌层浸润深度和分期有关。CTTN基因敲减后，EC细胞的迁移率、侵袭力和迁移能力均显著下降。观察到片状伪足消失，出现水泡。CTTN敲低后Rac 1蛋白表达降低。CTTN可促进片状伪足对EC的侵袭和迁移。这种作用可能与CTTN对Rac 1的调节有关。
The prognosis of endometrial cancer (EC) is significantly affected by tumor infiltration and metastasis. Cortactin (CTTN) regulates infiltration and metastasis in other tumors. Studies on the role and mechanism of CTTN in EC are limited and further studies are needed. Quantitative PCR and immunohistochemistry were used to detect Ras-associated C3 botulinum toxin substrate 1 (Rac1) and CTTN in EC and normal tissues. The relationship between the expression of these two genes and their prognostic factors was analyzed. A CTTN-RNAi lentiviral system was constructed and transfected into EC cells. Migration and invasion were evaluated by scratch assay, transwell migration, and invasion assays. Pseudopodia formation was observed by immunofluorescence staining. Western blotting was performed to detect the expression of Rac1. The expression levels of Rac1 and CTTN in EC tissues were significantly higher than those in normal tissues. In the EC group, Rac1 and CTTN levels were correlated. The protein expression levels of Rac1 and CTTN were related to myometrial invasion and stage. After CTTN knockdown, the migration rate, invasiveness, and migratory ability of EC cells decreased significantly. Lamellipodia was observed to disappear with the appearance of blebs. Rac1 protein expression was decreased after CTTN knockdown. CTTN may promote the invasion and migration of EC by lamellipodia. This effect may be related to the regulation of Rac1 by CTTN.
原始癌基因PBF的促侵入作用是通过与Cortactin的相互作用调节的。
DOI：
10.1210/jc.2016-1932
发表时间：
2016-12
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
Watkins RJ;Imruetaicharoenchoke W;Read ML;Sharma N;Poole VL;Gentilin E;Bansal S;Bosseboeuf E;Fletcher R;Nieto HR;Mallick U;Hackshaw A;Mehanna H;Boelaert K;Smith VE;McCabe CJ
通讯作者：
McCabe CJ