Uptake of Cadmium by Different Cultivars of Brassica pekinens (Lour.) Rupr. and Brassica chinensis L. and Their Potential for Phytoremediation
Uptake of Cadmium by Different Cultivars of Brassica pekinens (Lour.) Rupr. and Brassica chinensis L. and Their Potential for Phytoremediation
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DOI：
10.1007/s00128-006-0981-3
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发表时间：
2006-04
影响因子：
2.7
通讯作者：
C. P. Liu;Z. Shen;X. Li
中科院分区：
文献类型：
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作者：
C. P. Liu;Z. Shen;X. Li
材料与方法采样于南京农业大学农田0-30 cm土层。将土壤风干，粉碎以通过4 mm直径的筛子，然后阿尔蒂正式修正为100 mg kg-1干土壤Cd，以CdCl 2的形式存在。镉修正的土壤允许进行两个星期的水饱和和空气干燥周期。将风干的土壤（1000 g）置于塑料盆中。施入土壤的基肥为100 mg N kg-1干土尿素、80 mg P kg-1和100 mg K kg-1 KHZPO 4（Shen等人，2002年）。约15粒B种子。pekinensis（10个品种）和B.每盆播种10个品种。发芽后，将幼苗稀疏至每盆五株。花盆每天浇水至田间水量的65%。每个品种重复三次。在自然光下生长36天后，切下芽，用自来水彻底洗涤，然后用去离子水冲洗。将植物样品在80 ℃下干燥72小时.测量植物的干重。将植物材料在红玉髓研钵中研磨并用HNO 3-HC 1 O 4（87：13 v/v）的混合物酸消化。溶液中Cd的浓度通过TAS-986型号的HLAME原子吸收光谱仪（Purkinje General Co. Ltd.，北京）。使用美国国家标准与技术研究所认证的艾德标准参考物质（番茄叶）来验证金属分析的准确性。在适当的情况下，还使用试剂空白和分析重复样品，以确保分析的准确度和精密度。B的种子。pekirzensis（cv.交白6号、清研87-114、夏抗50、夏旺）用0.5%NaClO表面消毒，去离子水冲洗后，萌发3d。之后，将20株幼苗转移到含有Hoagland营养液的2dm 3盆中。将幼苗在自然光下在绿色室内生长30天。用ImM I-ICl或ImM NaOH将营养液的pH调节至6.0。营养液每三天更换一次。将CdCl 2以0和50 μ M的浓度加入溶液中。每个处理重复三次。在Cd处理后7 d收获枝条。将植物材料洗涤，在80 ℃下干燥72小时并称重，然后在红玉髓研钵中研磨。如前所述，用原子吸收光谱仪测定植物中的Cd浓度。
MATERIALS AND METHODSSoil samples were collected from the 0-30 cm layer of an agricultural field at Nanjing Agriculture University. Soils were air-dried, crushed to pass through a 4 mm diameter sieve, and then artificially amended to 100 mg kg'1 dry soil Cd in the form of CdCl2. The Cd-amended soils were allowed to undergo water saturation and air-drying cycles for two weeks. The air-dried soils (1 000 g) were placed in plastic pots. Basal fertilizers applied to the soils were 100 mg N kg'1 dry soil as urea, 80 mg P kg'1 and 100 mg K kg'las KHZPO4 (Shen et al. 2002). About 15 seeds of B. pekinensis (10 cultivars) and B. chinensis (10 cultivars) were sown in each pot. After germination, the seedlings were thinned to five plants per pot. The pots were watered daily to 65% of the field water capacity. Each cultivar was replicated three times. After 36 days growth under natural light, the shoots were cut, washed with tap water thoroughly, and then rinsed with de—ionized water. The samples of plants were dried at 80 C for 72 h. The dry weights of the plants were measured. The plant materials were ground in a carnelian mortar and were acid-digested with a mixture of HNO3-HCIO4 (87: 13 v/v). The concentrations of Cd in the solutions were determined by a flame atomic absorption spectrometer model TAS-986 (Purkinje General Co. Ltd., Beijing). A certified standard reference material (tomato leaves) of the National Institute of Standards and Technology, USA, was used to verify the accuracy of metal analysis. Reagent blank and analytical duplicates were also used where appropriate to ensure accuracy and precision in the analysis.Seeds of B. pekirzensis (cv. Jiaobai-6, Qingyan 87-114, Xiakang-50, Xiawang) were surface~ sterilized in 0.5% NaClO and rinsed thoroughly with de-ionized water, then germinated for 3 d. Afterwards, 20 seedlings were transferred to 2 dm3 pots containing Hoagland nutrient solutions. The seedlings were grown in a green house under natural light for 30 days. The pH of nutrient solutions was adjusted to 6.0 with 1 mM I-ICl or 1 mM NaOH. Nutrient solutions were renewed every three days. The CdCl2 was added into solution at the concentration of 0 and 50 uM. Each treatment was replicated three times. The shoots were harvested 7 d after the Cd treatment. The plant materials were washed, dried at 80 C for 72 h and weighted, and then were ground in a carnelian mortar. The Cd concentrations in plants were determined with a flame atomic absorption spectrometer as previously described.