Fecal Progestagen Evaluations to Monitor the Estrous Cycle and Pregnancy in the Okapi (Okapia johnstoni)
Fecal Progestagen Evaluations to Monitor the Estrous Cycle and Pregnancy in the Okapi (Okapia johnstoni)
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粪便孕激素评估以监测霍加皮 (Okapia johnstoni) 的发情周期和怀孕情况
DOI：
10.1002/zoo.1430120606
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发表时间：
1993
期刊：
影响因子：
1.3
通讯作者：
W. Meurichy
中科院分区：
文献类型：
--
作者：
F. Schwarzenberger;M. Patzl;R. Francke;A. Ochs;R. Buiter;W. Schaftenaar;W. Meurichy
本研究旨在建立一种非侵入性的霍加皮（Okapia johnstoni）生殖监测方法。每周三次收集粪便样本，从非怀孕的霍加匹斯（n = 3）的2个月，9个月和23个月，分别为2个月，每个怀孕的霍加匹斯（n = 4）在不同的妊娠阶段。使用抗孕激素抗体，用酶免疫分析法（EIA）分析样品，结果被认为是未结合的总免疫反应性孕激素（Pd-Pgs）的测量结果。在发情周期的卵泡期（FP）和黄体期（LP），Pd-Pgs的平均值分别为0.6 ± 0.1 μg/g和6.1 - 0.3 μg/g粪便。在LP的终端处发生配对。通过将整个LP的数量除以样品可用的时间，估计两种霍加的平均发情周期长度分别为15.5（n = 11 LP）和15.8（n = 36 LP）天。在3只动物中，通过观察到的交配计算出妊娠时间为423、424和431天。在分娩前-280 ~-220日龄，粪便Pd-Pgs含量在20--60 μg/g粪便范围内持续增加。在妊娠的最后三分之一期间，值为100- 350 μg/g。在分娩前一周内数值下降，产后3- 4天内观察到FP值持续下降。在1只动物产后2个月的研究期间，除了1只动物产后3周的LP外，Pd-Pgs均在FP范围内。得出的结论是，在FP、LP和妊娠后期，雌性霍加蜂的粪便Pd-Pgs分别以100：10：>100的比例存在。通过EIA测量它们可以无创地监测发情周期和妊娠诊断。© 1993 Wiley-Liss公司。
The present study was conducted to establish a noninvasive method of reproductive monitoring in the okapi (Okapia johnstoni). Fecal samples were collected three times a week from nonpregnant okapis (n = 3) for periods of 2, 9, and 23 months, respectively, and for 2 months each from pregnant okapis (n = 4) at different stages of gestation. Samples were analyzed with an enzyme-immunoas-say (EIA), using an antibody against pregnanediol, and the results are considered as measurements of unconjugated total immunoreactive progestagens (Pd-Pgs). Mean values of Pd-Pgs during the follicular (FP) and the luteal phases (LP) of the estrous cycles were 0.6 ± 0.1 μg/g and 6.1 −0.3 μg/g feces, respectively. Matings occurred at the terminations of the LP. By dividing the number of entire LPs into the time over which samples were available, average estrous cycle lengths in two okapis were estimated to be 15.5 (n = 11 LP) and 15.8 (n = 36 LP) days, respectively. In three animals, gestation lengths of 423, 424, and 431 days were calculated by observed matings. During days -280 to -220 before parturition, the fecal Pd-Pgs constantly increased from about 20--60 μg/g feces. Values were 100--350 μg/g during the last third of gestation. Values decreased in the week before parturition, and a continuous decline to FP values was observed within 3--4 days postpartum. During the 2 months postpartum investigated in one animal, the Pd-Pgs were in the FP range except one LP 3 weeks postpartum. It was concluded that fecal Pd-Pgs in female okapis are present in a ratio of ∼1: 10:>100 during FP, LP, and late pregnancy, respectively. Their measurement by EIA enables noninvasive monitoring of the estrous cycle and pregnancy diagnosis. © 1993 Wiley-Liss, Inc.
快速提取粪便类固醇，用于测量自由放养的黄色狒狒（Papio cynocephalus cynocephalus）的生殖周期和早期妊娠。
DOI：
10.1530/jrf.0.0920415
发表时间：
1991
期刊：
Journal of reproduction and fertility
影响因子：
--
作者：
Wasser,SK;Monfort,SL;Wildt,DE
通讯作者：
Wildt,DE