Improvement of porous β-TCP scaffolds with rhBMP-2 chitosan carrier film for bone tissue application
Improvement of porous β-TCP scaffolds with rhBMP-2 chitosan carrier film for bone tissue application
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DOI：
10.1089/ten.tea.2007.0229
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发表时间：
2008-08-01
影响因子：
4.1
通讯作者：
Lopez-Lacomba, Jose Luis
中科院分区：
文献类型：
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作者：
Abarrategi, Ander;Moreno-Vicente, Carolina;Lopez-Lacomba, Jose Luis
陶瓷材料是骨组织工程中广泛应用的骨传导基质材料。这些类型的生物材料的性能可以通过加入生物活性物质和材料而大大提高。此前有报道称，壳聚糖是一种生物相容、可生物降解的材料，可促进骨形成。另一方面，骨形态发生蛋白-2(BMP-2)是一种众所周知的骨诱导因子。在这项工作中，我们用重组人骨形态发生蛋白-2(rhBMP-2)载体壳聚糖薄膜包覆了多孔的β-磷酸三钙(β-TCP)支架，并研究了它们对陶瓷的物理化学性质、细胞响应和体内成骨的影响。将平均直径为5.78 mm、厚度为2.9 mm、孔隙率为53%的初始β-TCP圆盘涂覆一层壳聚糖膜。用X射线衍射仪、傅里叶变换红外光谱仪、透射电子显微镜、扫描电子显微镜和能量色散X射线分析(EDX)对涂层的性能进行了研究。处理改善了支架的孔洞分布，提高了平均硬度。生物相容性似乎没有改变。此外，由于重组人骨形态发生蛋白-2的掺入，贴壁的C2C12细胞表达了碱性磷酸酶活性，这与细胞向成骨方向分化有关。另一方面，体内观察显示，术后3周有新骨形成，比对照β-磷酸三钙陶瓷的时间要短得多。这些结果表明，开发的涂层改善了骨组织应用的多孔β-磷酸三钙支架，并增加了骨诱导性能。
Ceramic materials are osteoconductive matrices extensively used in bone tissue engineering approaches. The performance of these types of biomaterials can be greatly enhanced by the incorporation of bioactive agents and materials. It is previously reported that chitosan is a biocompatible, biodegradable material that enhances bone formation. In the other hand, bone morphogenetic protein-2 (BMP-2) is a well-known osteoinductive factor. In this work we coated porous beta-tricalcium phosphate (beta-TCP) scaffolds with recombinant human BMP-2 (rhBMP-2) carrier chitosan films and studied how they could modify the ceramic physicochemical properties, cellular response, and in vivo bone generation. Initial beta-TCP disks with an average diameter of 5.78 mm, 2.9mm thickness, and 53% porosity were coated with a chitosan film. These coating properties were studied by X-ray diffraction, Fourier transform-infrared analysis, transmission electron microscopy, scanning electron microscopy, and energy dispersive X-ray analysis (EDX). Treatment modified the scaffold porous distribution and increased the average hardness. The biocompatibility did not seem to be altered. In addition, adhered C2C12 cells expressed alkaline phosphatase activity, related to cell differentiation toward osteogenic lineage, due to the incorporation of rhBMP-2. On the other hand, in vivo observations showed new bone formation 3 weeks after surgery, a much shorter time than control beta-TCP ceramics. These results suggest that developed coating improved porous beta-TCP scaffold for bone tissue applications and added osteoinductive properties.