Molecular detection of Bacteroides forsythus in human periodontitis.
Molecular detection of Bacteroides forsythus in human periodontitis.
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人牙周炎中福赛斯拟杆菌的分子检测。
DOI：
10.1111/j.1399-302x.1994.tb00052.x
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发表时间：
1994
影响因子：
--
通讯作者：
Slots,J
中科院分区：
文献类型：
--
作者：
Lotufo,RF;Flynn,J;Chen,C;Slots,J
研究了地高辛标记的基因组DNA探针检测龈下拟杆菌的有效性。此外，还采用随机引物聚合酶链反应（AP-PCR）技术对B.因此牙周分离物。DNA探针检测到103 B。在104个细胞处产生强信号。它与B反应。结果表明，该菌株与ATCC 43037 T和44株临床分离株无反应，与其他24种口腔微生物的75株菌株无反应。与培养相比，斑点印迹法中的DNA探针显示出88.8%的灵敏度和38.4%的特异性（准确性，72.5%）。通过在原代平板上进行菌落印迹，获得了98.1%的灵敏度和53.8%的特异性（准确性，82.5%i）。在614例牙周炎患者中，449例（73.1%）检测到这种情况。与牙龈卟啉单胞菌密切相关，两者共检出54.8%。在270个研究样本中，22.2%的样本不存在。AP-PCR鉴定为24 B。结果表明，27株供试菌株的基因型为：这项研究证明了非放射性基因组探针直接检测B的实用性。forsvthusin龈下标本。该物种表现出相当程度的遗传多样性。DNA分析可能有助于确定B.暴露个体中的嗜酸乳杆菌牙周病及其传播方式。
The usefulness of a digoxigenin‐labeled genomic DNA probe for the detection of subgingivalBacteroides forsythuswas examined. In addition, the arbitrarily primed polymerase chain reaction (AP‐PCR) was used to delineate the genetic diversity ofB. forsythusperiodontal isolates. The DNA probe detected 103B. forsvthuscells and yielded a strong signal at 104cells. It reacted withB. forsythusATCC 43037Tand 44 clinical isolates and showed no detectable reactivity with 75 strains of 24 other oral microbial species. In comparison to culture, the DNA probe in a dot‐blot method demonstrated a sensitivity of 88.8% and a specificity of 38.4% (accuracy, 72.5%). By colony‐blotting on primary plates, a sensitivity of 98.1% and a specificity of 53.8% (accuracy, 82.5%i) were obtained.B. forsythuswas detected in 449 (73.1%) of 614 periodontitis patients. The occurrence of the organism was closely associated withPorphyromonas gingivalis, both species being present in 54.8%. and absent in 22.2%, of 270 study samples. AP‐PCR identified 24B. forsythusgenotypes among 27 test strains. This study demonstrated the utility of a non‐radioactive genomic probe for direct detection ofB. forsvthusin subgingival specimens. The species showed a considerable degree of genetic diversity. DNA analysis may help to determine the role ofB. forsythusin periodontal disease and its mode of transmission among exposed individuals.