Solubility phase transition of maternal RNAs during vertebrate oocyte-to-embryo transition
Solubility phase transition of maternal RNAs during vertebrate oocyte-to-embryo transition
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DOI：
10.1016/j.devcel.2023.10.005
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发表时间：
2023-12-04
影响因子：
11.8
通讯作者：
Yang，Jing
中科院分区：
文献类型：
--
作者：
Hwang，Hyojeong;Chen，Sijie;Yang，Jing
卵母细胞向胚胎转变（OET）受储存在卵母细胞质中的母体产物的调节，不依赖于转录。母体产品如何精确地重塑以决定OET在很大程度上仍不清楚。在这项工作中，我们发现了母体RNA在XenopusOET过程中的动态溶解度相变。我们已经确定了863个母转录本，从一个可溶性的状态过渡到一个洗涤剂不溶性的卵母细胞成熟后。这些RNA在动物大脑半球中富集，其中许多编码关键的细胞周期调节因子。与此相反，165个转录本，包括几乎所有的Xenopusgermline RNA和一些植物定位的体细胞RNA，经历了不溶性到可溶性的相变。这种现象在斑马鱼中是保守的。我们的结果表明，种系RNA的相变影响其对RNA降解机制的易感性，并且是由种质的重塑介导的。因此，这项工作确定了作用于RNA以控制脊椎动物OET的重要重塑机制。
The oocyte-to-embryo transition (OET) is regulated by maternal products stored in the oocyte cytoplasm, independent of transcription. How maternal products are precisely remodeled to dictate the OET remains largely unclear. In this work, we discover the dynamic solubility phase transition of maternal RNAs duringXenopusOET. We have identified 863 maternal transcripts that transition from a soluble state to a detergent-insoluble one after oocyte maturation. These RNAs are enriched in the animal hemisphere, and many of them encode key cell cycle regulators. In contrast, 165 transcripts, including nearly allXenopusgermline RNAs and some vegetally localized somatic RNAs, undergo an insoluble-to-soluble phase transition. This phenomenon is conserved in zebrafish. Our results demonstrate that the phase transition of germline RNAs influences their susceptibility to RNA degradation machinery and is mediated by the remodeling of germ plasm. This work thus identifies important remodeling mechanisms that act on RNAs to control vertebrate OET.