Absolute Quantification of Uric Acid in Human Urine Using Surface Enhanced Raman Scattering with the Standard Addition Method.
Absolute Quantification of Uric Acid in Human Urine Using Surface Enhanced Raman Scattering with the Standard Addition Method.
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DOI：
10.1021/acs.analchem.6b04588
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发表时间：
2017-02
影响因子：
7.4
通讯作者：
C. Westley;Yun Xu;B. Thilaganathan;A. Carnell;N. Turner;R. Goodacre
中科院分区：
文献类型：
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作者：
C. Westley;Yun Xu;B. Thilaganathan;A. Carnell;N. Turner;R. Goodacre
尿液和血清中的高水平尿酸可以指示高血压和妊娠相关病症，先兆子痫。我们开发了一种简单、经济、便携式的表面增强拉曼散射（Sers）方法，用于患者尿样中临床相关水平的尿酸的常规分析。这种方法与标准加入法（SAM）相结合，可以直接在复杂基质（如人尿液）中对尿酸进行绝对定量。结果具有高度可比性，并且与HPLC结果非常一致，在所有分析的样品中，两种分析方法之间的预测值平均差异<9%，与HPLC相比，Sers的采集时间减少了60倍。临床子痫患者样本首次被用于使用简单，快速的胶体Sers方法进行定量尿酸检测，而无需复杂的数据分析。
High levels of uric acid in urine and serum can be indicative of hypertension and the pregnancy related condition, preeclampsia. We have developed a simple, cost-effective, portable surface enhanced Raman scattering (SERS) approach for the routine analysis of uric acid at clinically relevant levels in urine patient samples. This approach, combined with the standard addition method (SAM), allows for the absolute quantification of uric acid directly in a complex matrix such as that from human urine. Results are highly comparable and in very good agreement with HPLC results, with an average <9% difference in predictions between the two analytical approaches across all samples analyzed, with SERS demonstrating a 60-fold reduction in acquisition time compared with HPLC. For the first time, clinical prepreeclampsia patient samples have been used for quantitative uric acid detection using a simple, rapid colloidal SERS approach without the need for complex data analysis.