Cytokine responses in birds challenged with the human food-borne pathogen Campylobacter jejuni implies a Th17 response.
Cytokine responses in birds challenged with the human food-borne pathogen Campylobacter jejuni implies a Th17 response.
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DOI：
10.1098/rsos.150541
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发表时间：
2016-03
影响因子：
3.5
通讯作者：
Rushton SP
中科院分区：
文献类型：
--
作者：
Reid WD;Close AJ;Humphrey S;Chaloner G;Lacharme-Lora L;Rothwell L;Kaiser P;Williams NJ;Humphrey TJ;Wigley P;Rushton SP
在对病原体的免疫应答期间，胃肠道内细胞因子相互作用的过程导向理解的发展需要实验和统计建模。针对病原体攻击的免疫应答取决于对宿主的特定威胁。在这里，我们表明，肉鸡安装一个品种依赖性的免疫反应空肠弯曲菌感染盲肠通过分析实验数据，使用频率和贝叶斯结构方程模型（SEM）。SEM提供了一个框架，通过该框架，可以基于先验知识来测试细胞因子的相互依赖性。在两个品种中，重要的细胞因子包括促炎性白细胞介素（IL）-1β、IL-4、IL-17 A、干扰素（IFN）-γ和抗炎性IL-10和转化生长因子（TGF）-β4在攻击后表达。SEM揭示了一个假定的调节途径，说明了T辅助细胞（Th）17的反应和调节IL-10，这是品种依赖性。Th 17途径的突出表明细胞因子应答旨在限制细胞外细菌病原体的入侵或定殖，但应答的时间依赖性在品种之间不同。
Development of process orientated understanding of cytokine interactions within the gastrointestinal tract during an immune response to pathogens requires experimentation and statistical modelling. The immune response against pathogen challenge depends on the specific threat to the host. Here, we show that broiler chickens mount a breed-dependent immune response to Campylobacter jejuni infection in the caeca by analysing experimental data using frequentist and Bayesian structural equation models (SEM). SEM provides a framework by which cytokine interdependencies, based on prior knowledge, can be tested. In both breeds important cytokines including pro-inflammatory interleukin (IL)-1β, , IL-4, IL-17A, interferon (IFN)-γ and anti-inflammatory IL-10 and transforming growth factor (TGF)-β4 were expressed post-challenge. The SEM revealed a putative regulatory pathway illustrating a T helper (Th)17 response and regulation of IL-10, which is breed-dependent. The prominence of the Th17 pathway indicates the cytokine response aims to limit the invasion or colonization of an extracellular bacterial pathogen but the time-dependent nature of the response differs between breeds.