Marked induction of inducible nitric oxide synthase and tumor necrosis factor-α in rat CD40+ microglia by comparison to CD40- microglia
Marked induction of inducible nitric oxide synthase and tumor necrosis factor-α in rat CD40+ microglia by comparison to CD40- microglia
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DOI：
10.1016/j.jneuroim.2009.01.007
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发表时间：
2009-03-31
影响因子：
3.3
通讯作者：
Nakayama, Hitoshi
中科院分区：
文献类型：
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作者：
Kawahara, Kohichi;Yoshida, Akira;Nakayama, Hitoshi
至少在中枢神经系统中可能存在两种小胶质细胞（MG）亚型。我们从大鼠混合胶质细胞培养中分离出两种类型。LPS刺激后，诱导型一氧化氮合酶（iNOS）和肿瘤坏死因子- α (TNF - α) mrna和蛋白在CD40(+) MG中被诱导的程度高于CD40(-) MG。尽管LPS受体的表达水平在不同亚型之间差异不大，但在CD40(+) MG中，LPS诱导的磷酸化I κ pa B α的降解被标记。这些结果强烈提示CD40(+) MG在一定病理条件下产生大量NO和TNF α，表现出神经毒性作用。(C) 2009 Elsevier B.V.版权所有
There may be two subtypes of microglia (MG) at least in the CNS. We separated the two types from rat mixed glial culture. mRNAs and proteins for inducible nitric oxide synthase (iNOS) and tumor necrosis factor-alpha (TNF alpha) were more induced in CD40(+) MG than CD40(-) MG after LPS stimulation. Although the expression level of LPS receptors showed a little difference between the subtypes, LPS-induced degradation of phosphorylated I kappa B alpha was marked in CD40(+) MG. These results strongly suggest that CD40(+) MG produce larger amount of NO and TNF alpha to exhibit neurotoxic action under certain pathological conditions in brains. (C) 2009 Elsevier B.V. All rights reserved.