Native Mass Spectrometry Characterization of Intact Nanodisc Lipoprotein Complexes
Native Mass Spectrometry Characterization of Intact Nanodisc Lipoprotein Complexes
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DOI：
10.1021/ac302663f
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发表时间：
2012-11-06
影响因子：
7.4
通讯作者：
Sligar, Stephen G.
中科院分区：
文献类型：
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作者：
Marty, Michael T.;Zhang, Hao;Sligar, Stephen G.
我们在这里描述了通过使用原生质谱（MS）来表征纳米盘复合物的分子量（MW）和多分散性的分析。纳米圆盘是纳米级脂质双层，为溶解膜蛋白提供了平台。与洗涤剂胶束不同，纳米圆盘是类似天然的脂质双层，其界限明确且可能是单分散的。它们的质谱允许根据每个复合物中单个脂质的质量差异进行峰分配。所得质量与预测值非常吻合，并最终证明了纳米盘中脂质分子的窄分散性。碰撞激活解离 (CAD) 或电子捕获解离 (ECD) 的断裂显示少量脂质的损失，以及随着支架蛋白的释放，纳米盘最终崩溃。这些结果为未来利用纳米圆盘作为将膜蛋白发射到气相的平台的研究奠定了基础。
We describe here the analysis of nanodisc complexes by using native mass spectrometry (MS) to characterize their molecular weight (MW) and polydispersity. Nanodiscs are nanoscale lipid bilayers that offer a platform for solubilizing membrane proteins. Unlike detergent micelles, nanodiscs are native-like lipid bilayers that are well-defined and potentially monodisperse. Their mass spectra allow peak assignment based on differences in the mass of a single lipid per complex. Resultant masses agree closely with predicted values and demonstrate conclusively the narrow dispersity of lipid molecules in the nanodisc. Fragmentation with collisionally activated dissociation (CAD) or electron capture dissociation (ECD) shows loss of a small number of lipids and eventual collapse of the nanodisc with release of the scaffold protein. These results provide a foundation for future studies utilizing nanodiscs as a platform for launching membrane proteins into the gas phase.