Parathyroid hormone blocks the stimulatory effect of insulin-like growth factor-I on collagen synthesis in cultured 21-day fetal rat calvariae.
Parathyroid hormone blocks the stimulatory effect of insulin-like growth factor-I on collagen synthesis in cultured 21-day fetal rat calvariae.
复制标题
甲状旁腺激素阻断胰岛素样生长因子-I 对培养的 21 天胎鼠颅盖胶原蛋白合成的刺激作用。
DOI：
10.1016/8756-3282(90)90136-m
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发表时间：
1990
期刊：
影响因子：
4.1
通讯作者：
Raisz,LG
中科院分区：
文献类型：
--
作者：
Kream,BE;Petersen,DN;Raisz,LG
通过测定[3H]脯氨酸在胶原酶可消化蛋白中的掺入量，研究了甲状旁腺激素（PTH）和重组人胰岛素样生长因子I （IGF-I）对21天胎鼠颅骨胶原合成的相互作用。培养96小时后，10 nM PTH拮抗胶原合成刺激，部分阻断10 nM IGF-I产生的干重增加。甲状旁腺激素（PTH）阻断igf - 1刺激的颅骨中央骨胶原合成的作用可被福斯柯林和12肉豆蔻酸13-醋酸佛波酯模拟，但1,25-二羟基维生素D3、转化生长因子-α和地塞米松不具有这种作用。我们的数据与PTH的直接作用是抑制基础CDP标记并完全反对IGF-I刺激的CDP标记的概念一致。发现PTH的这种作用被forskolin和PMA模拟，这表明IGF-I刺激CDP标记的阻断涉及cAMP和蛋白激酶C介导的途径。
We examined the interaction of parathyroid hormone (PTH) and recombinant human insulin-like growth factor I (IGF-I) on collagen synthesis in 21-day fetal rat calvariae as assessed by measuring the incorporation of [3H]proline into collagenase-digestible protein. After 96 hours of culture, 10 nM PTH antagonized the stimulation of collagen synthesis and partially blocked the increase in dry weight produced by 10 nM IGF-I. The effect of PTH to block IGF-I stimulated collagen synthesis was observed in the central bone of calvariae and was mimicked by forskolin and phorbol 12myristate 13-acetate, but not by 1,25-dihydroxyvitamin D3, transforming growth factor-α or dexamethasone. Our data are consistent with the concept that the direct effect of PTH is to inhibit basal CDP labeling and fully oppose IGF-I stimulated CDP labeling. The finding that this effect of PTH is mimicked by forskolin and PMA suggests that this block in IGF-I stimulation of CDP labeling involves both cAMP and protein kinase C mediated pathways.