A homeodomain protein binds to gamma-globin gene regulatory sequences.
A homeodomain protein binds to gamma-globin gene regulatory sequences.
复制标题
同源结构域蛋白与γ-珠蛋白基因调控序列结合。
DOI：
10.1073/pnas.88.16.7318
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发表时间：
1991
影响因子：
11.1
通讯作者：
DeSimone,J
中科院分区：
文献类型：
--
作者：
Lavelle,D;Ducksworth,J;Eves,E;Gomes,G;Keller,M;Heller,P;DeSimone,J
Developmental regulation of gamma-globin gene expression probably occurs through developmental-stage-specific trans-acting factors able to promote the interaction of enhancer elements located in the far upstream locus control region with regulatory elements in the gamma gene promoters and 3' A gamma enhancer located in close proximity to the genes. We have detected a nuclear protein in K562 and baboon fetal bone marrow nuclear extracts capable of binding to A+T-rich sequences in the locus control region, gamma gene promoter, and 3' A gamma enhancer. SDS/polyacrylamide gel analysis of the purified K562 binding activity revealed a single protein of 87 kDa. A K562 cDNA clone was isolated encoding a beta-galactosidase fusion protein with a DNA binding specificity identical to that of the K562/fetal bone marrow nuclear protein. The cDNA clone encodes a homeodomain homologous to the Drosophila antennapedia protein.
