Cell cycle programs of gene expression control morphogenetic protein localization.
Cell cycle programs of gene expression control morphogenetic protein localization.
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DOI：
10.1083/jcb.151.7.1501
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发表时间：
2000-12-25
期刊：
影响因子：
--
通讯作者：
Chant J
中科院分区：
文献类型：
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作者：
Lord M;Yang MC;Mischke M;Chant J
酵母基因组研究表明，八分之一的基因在其表达中受到细胞周期的调控。几乎无一例外地，细胞周期周期性基因表达的意义尚未得到检验。鉴于许多这样的基因对细胞形态发生至关重要，我们想研究周期性基因表达对这一过程的重要性。细胞分裂轴向模式所需的两个基因BUD 3和BUD 10/AXL 2/SRO 4的表达谱受到细胞周期的强烈调控。BUD 3在接近有丝分裂开始时表达。BUD 10在G1晚期表达。通过启动子交换实验，改变了每个基因的表达谱，并检查了结果。我们发现BUD 3表达的S/G2脉冲控制Bud 3 p定位的时间，但是该时间对于Bud 3 p功能不是关键的。相反，BUD 10表达的G1脉冲在Bud 10 p定位和功能中起直接作用。Bud 10 p是一种膜蛋白，依赖于G1特异性的极化分泌机制被递送到其适当的位置。这种基于分泌的膜蛋白靶向机制为细胞提供了重塑其结构或进化新形式的灵活性。
Genomic studies in yeast have revealed that one eighth of genes are cell cycle regulated in their expression. Almost without exception, the significance of cell cycle periodic gene expression has not been tested. Given that many such genes are critical to cellular morphogenesis, we wanted to examine the importance of periodic gene expression to this process. The expression profiles of two genes required for the axial pattern of cell division, BUD3 and BUD10/AXL2/SRO4, are strongly cell cycle regulated. BUD3 is expressed close to the onset of mitosis. BUD10 is expressed in late G1. Through promotor-swap experiments, the expression profile of each gene was altered and the consequences examined. We found that an S/G2 pulse of BUD3 expression controls the timing of Bud3p localization, but that this timing is not critical to Bud3p function. In contrast, a G1 pulse of BUD10 expression plays a direct role in Bud10p localization and function. Bud10p, a membrane protein, relies on the polarized secretory machinery specific to G1 to be delivered to its proper location. Such a secretion-based targeting mechanism for membrane proteins provides cells with flexibility in remodeling their architecture or evolving new forms.