CEREBROSPINAL-FLUID GLUCOSE - TURNOVER AND METABOLISM
CEREBROSPINAL-FLUID GLUCOSE - TURNOVER AND METABOLISM
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DOI：
10.1111/j.1471-4159.1985.tb07176.x
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发表时间：
1985-01-01
影响因子：
4.7
通讯作者：
FERGUSON, V
中科院分区：
文献类型：
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作者：
HOCHWALD, GM;MAGEE, J;FERGUSON, V
研究了猫在稳态灌流过程中脑脊液葡萄糖的周转情况。在所有实验中，灌流液中要么只含有示踪剂[14C]葡萄糖，要么含有示踪剂葡萄糖和4.45mM未标记葡萄糖。在一些研究中，胰岛素可以降低血糖。测定流出液中葡萄糖和[~(14)C]葡萄糖的浓度，用层析法测定~(14)C在葡萄糖和乳酸之间的分配。计算了葡萄糖的提取和葡萄糖代谢为乳酸的量。同时测定了血清葡萄糖的内流。在稳态灌流期间，71%的放射性在流出液中恢复：50%以葡萄糖的形式，6%以乳酸的形式，15%以未鉴定的形式。因此，50%的灌流液葡萄糖被清除，其中29%被提取，21%被代谢。当后者为apprx时，葡萄糖内流与血糖成正比。2.5mM或10.0mM，仅用示踪葡萄糖灌流时，流出液中的葡萄糖浓度为血清的25%。血清中葡萄糖的转运与血清和灌注液之间的葡萄糖浓度梯度无关。当灌注液葡萄糖浓度大于血清葡萄糖浓度时，转运受到抑制。在维持脑脊液葡萄糖浓度低于血清葡萄糖的情况下，在进入和离开脑脊液的葡萄糖相同的情况下，清除的50%的脑脊液葡萄糖浓度被25%的血清葡萄糖浓度所取代。
The turnover of CSF glucose was studied in cats during steady-state perfusion. In all experiments, the perfusion fluid contained either tracer [14C] glucose alone or tracer glucose along with 4.45 m M unlabeled glucose. In some studies, serum glucose was lowered with insulin. The concentration of glucose and [14C] glucose in the effluent fluid was measured, and the distribution of 14C between glucose and lactate was determined by chromatography. The extraction of glucose and the metabolism of glucose to lactate were calculated. The influx of glucose from serum was also determined. During steady-state perfusion, 71% of the radioactivity was recovered in the effluent fluid: 50% in the form of glucose, 6% in the form of lactate and 15% in forms that were not identified. Thus, 50% of the perfusion fluid glucose was cleared, of which 29% was extracted and 21% metabolized. The influx of glucose was proportional to the serum glucose when the latter .apprx. 2.5 m M or 10.0 m M. During perfusion with tracer glucose only, the concentration of glucose in the effuent fluid was 25% that of serum. The transport of glucose from serum was independent of the glucose concentration gradient between serum and perfusion fluid. When perfusion fluid glucose concentration was greater than that of serum, transport was inhibited. In maintaining CSF glucose at a lower concentration than serum glucose, with equal amounts of glucose entering and leaving the CSF, 50% of CSF glucose concentration cleared is replaced by 25% of serum glucose concentration.