The chemical composition of animal cells reconstructed from 2D and 3D ToF-SIMS analysis
The chemical composition of animal cells reconstructed from 2D and 3D ToF-SIMS analysis
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DOI：
10.1016/j.apsusc.2008.05.289
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发表时间：
2008-12-15
影响因子：
6.7
通讯作者：
Hagenhoff, B.
中科院分区：
文献类型：
--
作者：
Breitenstein, D.;Rommel, C. E.;Hagenhoff, B.
本文综述了近两年来在飞行时间二次离子质谱（ToF-SIMS）分析单细胞和类组织细胞层方面取得的进展。基本上进行了两种类型的调查：一方面是二维成像，另一方面是三维微区分析。在这两种情况下，化学固定结合缓慢的空气干燥被用作简单的样品制备方法。这两种方法的目标是确定天然成分的分布以及异生荧光团的定位。在我们的实验装置中，磷脂酰胆碱和氨基酸信号的分布符合预期。相比之下，荧光体乙锭同二聚体的分布只能在二维成像中检测到，而在三维分析中检测不到。另外四种荧光体在后一种方法中也失败了。因此，在我们的手中，三维检测到目前为止仅限于某些分子与一个非常低的质量和/或固有的电荷。(C)2008年爱思唯尔B。V.保留所有权利。
This paper gives an overview on the progress achieved in our lab within the last two years in the analysis of single cells and tissue-like cell layers by time-of-flight secondary ion mass spectrometry (ToF-SIMS). Basically two types of investigations were performed: on the one hand a two-dimensional imaging and on the other hand a three-dimensional microarea analysis. In both cases chemical fixation in combination with slow air-drying were used as easy sample preparation method. It was the goal of both approaches to identify the distribution of natural components as well as the localisation of xenobiotic fluorophors. In our experimental set-ups the distribution of phophatidylcholine and amino-acid signals were in line with the expectation. In contrast, the distribution of the fluorophor ethidiumhomodimer could only be detected within the two-dimensional imaging, whereas it was not detected in the three-dimensional analysis. Also four other fluorophors failed in the latter approach. Thus, in our hands the three-dimensional detection is to date limited to certain molecules with a comparably low mass and/or an intrinsical charge. (C) 2008 Elsevier B. V. All rights reserved.