Cleavage of folates during ethanol metabolism. Role of acetaldehyde/xanthine oxidase-generated superoxide.
Cleavage of folates during ethanol metabolism. Role of acetaldehyde/xanthine oxidase-generated superoxide.
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DOI：
10.1042/bj2570277
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
S. Shaw;E. Jayatilleke;V. Herbert;N. Colman
中科院分区：
文献类型：
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作者：
S. Shaw;E. Jayatilleke;V. Herbert;N. Colman
虽然在大多数酗酒者中观察到叶酸缺乏和对叶酸的需求增加，但乙醇代谢产生的乙醛可能会增加叶酸代谢的可能性尚未得到证实。在体外研究了黄嘌呤氧化酶代谢乙醛过程中叶酸的裂解，用高效液相色谱法测定叶酸对氨基苯甲酰谷氨酸的生成。乙醛/黄嘌呤氧化酶产生超氧化物，其裂解叶酸（5-甲基四氢叶酸大于亚叶酸大于叶酸），并被超氧化物歧化酶抑制。裂解增加铁蛋白的加入和抑制去铁胺（铁的紧密螯合剂），这表明催化铁的重要性。在体内黄嘌呤氧化酶存在下，乙醇代谢为乙醛产生的超氧化物可能导致酒精患者叶酸缺乏的严重性。
Although folate deficiency and increased requirements for folate are observed in most alcoholics, the possibility that acetaldehyde generated from ethanol metabolism may increase folate catabolism has not been previously demonstrated. Folate cleavage was studied in vitro during the metabolism of acetaldehyde by xanthine oxidase, measured as the production of p-aminobenzoylglutamate from folate using h.p.l.c. Acetaldehyde/xanthine oxidase generated superoxide, which cleaved folates (5-methyltetrahydrofolate greater than folinic acid greater than folate) and was inhibited by superoxide dismutase. Cleavage was increased by addition of ferritin and inhibited by desferrioxamine (a tight chelator of iron), suggesting the importance of catalytic iron. Superoxide generated from the metabolism of ethanol to acetaldehyde in the presence of xanthine oxidase in vivo may contribute to the severity of folate deficiency in the alcoholic.