Prevention of tumor growth by needle-free jet injection of anti-C7orf24 siRNA
Prevention of tumor growth by needle-free jet injection of anti-C7orf24 siRNA
复制标题
DOI：
10.1038/cgt.2012.31
复制
发表时间：
2012-08-01
影响因子：
6.4
通讯作者：
Kogure, K.
中科院分区：
文献类型：
--
作者：
Hama, S.;Arata, M.;Kogure, K.
通过蛋白质组分析鉴定的染色体7开放阅读框24（C7 orf 24）在各种类型的癌症中上调，并且与细胞增殖相关。然而，敲低C7orf24的体内抗肿瘤作用尚未阐明。本研究探讨了无针喷射注射（JI）抗C7orf24小干扰RNA（siRNA）对肺癌荷瘤小鼠的抗肿瘤作用。转染抗C7orf24 siRNA通过特异性敲低C7orf24诱导培养的人肺癌细胞的细胞毒性。此外，JI可以有效地将抗C7orf24 siRNA递送至肿瘤组织，并且因此显著抑制肿瘤生长。免疫组织化学分析显示，在从施用抗C7orf24 siRNA的小鼠收集的肿瘤组织中，C7orf24水平显著降低，表明C7orf24的敲低诱导了肿瘤组织中的细胞毒性。总之，这些数据首次表明，C7orf24的敲低在JI介导的siRNA递送后阻止体内肿瘤生长。
Chromosome 7 open reading frame 24 (C7orf24), which was identified by proteome analysis, is upregulated in various types of cancer and is associated with cellular proliferation. However, in vivo antitumor effect by knockdown of C7orf24 has not been clarified. In this study, we investigated that the antitumor effect of anti-C7orf24 small interfering RNA (siRNA) administered by needle-free jet injection (JI) on lung cancer-bearing mice. Transfection of anti-C7orf24 siRNA induced cytotoxicity in cultured human lung cancer cells through specific knockdown of C7orf24. Furthermore, JI could effectively deliver anti-C7orf24 siRNA to tumor tissues, and as a result tumor growth was significantly inhibited. Immunohistochemical analysis revealed that C7orf24 levels were significantly reduced within tumor tissues collected from anti-C7orf24 siRNA-administered mice, indicating that the knockdown of C7orf24 induced cytotoxicity in tumor tissue. In conclusion, these data show for the first time that knockdown of C7orf24 prevents tumor growth in vivo following JI-mediated the siRNA delivery.