Monitoring zearalenone in corn flour utilizing novel self-enhanced electrochemiluminescence aptasensor based on NGQDs-NH2-Ru@SiO2 luminophore
Monitoring zearalenone in corn flour utilizing novel self-enhanced electrochemiluminescence aptasensor based on NGQDs-NH2-Ru@SiO2 luminophore
复制标题
利用基于 NGQDs-NH2-Ru@SiO2 发光体的新型自增强电化学发光适体传感器监测玉米粉中的玉米赤霉烯酮
DOI：
10.1016/j.foodchem.2019.04.050
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发表时间：
2019-09-15
期刊：
影响因子：
8.8
通讯作者：
You, Tianyan
中科院分区：
文献类型：
--
作者：
Luo, Lijun;Ma, Shuai;You, Tianyan
霉菌毒素的准确、早期诊断对食品和农产品安全尤为重要。本文基于一种新型的自增强电化学发光（ECL）感应传感器，建立了一种灵敏、有效的玉米赤霉烯酮（ZEN）监测方法。通过静电将胺功能化Ru(bpy)(3)（2+）掺杂二氧化硅纳米颗粒（NH2-Ru@SiO2 NPs）与氮掺杂石墨烯量子点（NGQDs）结合在一起，合成了自增强光团。由于发射极和共反应物同时存在于同一纳米粒子中，缩短了电子传递距离，减小了能量损失。因此，基于该新型配合物的自增强ECL感应传感器在ZEN检测中线性范围最宽为10 fg mL(-1)-10 ng mL(-1)，最低检测限为1 fg mL（-1）。更重要的是，利用该传感器对玉米粉霉变过程中产生的ZEN进行了监测，在实际样品中具有良好的检测效果和应用前景。
Accurate and early diagnosis of mycotoxin is particularly significant to the food and agricultural product safety. In the present work, a sensitive and effective monitoring method for zearalenone (ZEN) was exploited based on a novel self-enhanced electrochemiluminescence (ECL) aptasensor. The self-enhanced lumonophore was compounded by electrostatically combining amine-functionalized Ru(bpy)(3)(2+) -doped silica nanoparticles (NH2-Ru@SiO2 NPs) and nitrogen doped graphene quantum dots (NGQDs) together. Since the emitter and co-reactant simultaneously existed in the same nanoparticle, shortened electron-transfer distance and decreased energy loss was obtained. Therefore, self-enhanced ECL aptasensor based on the novel complex expressed the widest linear range of 10 fg mL(-1)-10 ng mL(-1) and the lowest detection limit of 1 fg mL(-1) for ZEN detection. More importantly, ZEN produced during the mildew process of corn flour was monitored by the developed aptasensor, which exhibited superior determination and potential application in real samples.