BerlinTrap: A new cryogenic 22-pole ion trap spectrometer
BerlinTrap: A new cryogenic 22-pole ion trap spectrometer
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DOI：
10.1016/j.jms.2016.08.017
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发表时间：
2017-02-01
影响因子：
1.4
通讯作者：
Dopfer, Otto
中科院分区：
文献类型：
--
作者：
Guenther, Alan;Nieto, Pablo;Dopfer, Otto
介绍了一种新型串联质谱计（BerlinTrap）的设计和首次应用，该串联质谱计由电喷雾离子源、四极质谱计、低温22极离子阱（4-300 K）和正交反射飞行时间质谱仪组成。被捕获的离子被氦缓冲气体冷却。弱结合的henh30 +配合物的形成和溶剂化壳结构以及质子化氨基酸酪氨酸的电子光解离光谱用于校准冷却，标记和光谱能力的设置。推断质子化酪氨酸的振动温度低于20 K。在可见范围内记录了最小的黄素质子化光色素的电子谱，并通过量子化学计算将其分配给最稳定的N5异构体。这些结果证明了BerlinTrap装置适用于小型和大型（生物）分子离子及其簇的光谱和反应性研究。(C) 2016 Elsevier Inc.版权所有。
The design and first applications of a new tandem mass spectrometer (BerlinTrap) combining an electrospray ion source, a quadrupole mass spectrometer, a cryogenic 22-pole ion trap (4-300 K), and an orthogonal reflectron time-of-flight mass spectrometer are described. The trapped ions are cooled by helium buffer gas cooling. The formation and solvation shell structure of weakly-bound HenH3O+ complexes and the electronic photodissociation spectrum of the protonated amino acid tyrosine are used to calibrate the setup for cooling, tagging, and spectroscopic capabilities. A vibrational temperature below 20 K is inferred for protonated tyrosine. The electronic spectrum of isolated protonated lumichrome, the smallest protonated flavin, is recorded in the visible range and assigned to the most stable N5 isomer by comparison with quantum chemical calculations. These results demonstrate the suitability of the BerlinTrap apparatus for spectroscopy and reactivity studies of small and large (bio-)molecular ions and their clusters. (C) 2016 Elsevier Inc. All rights reserved.