Relationship between the golgi apparatus, gerl, and secretory granules in acinar cells of the rat exorbital lacrimal gland
Relationship between the golgi apparatus, gerl, and secretory granules in acinar cells of the rat exorbital lacrimal gland
复制标题
大鼠眶外泪腺腺泡细胞高尔基体、凝胶和分泌颗粒的关系
作者：
A. Hand;C. Oliver
本文应用电镜形态学和细胞化学技术研究了大鼠眶外泪腺腺泡细胞分泌颗粒的形成方法。高尔基体内面的未成熟分泌颗粒经常附着于狭窄的脑池结构，类似于Novikoff等人在神经元中描述的GERL（Novikoff，P.M.，A. B。Novikoff，N. Quintana和J. - J. Hauw. 1971. J. Cell Bio. 50：859-886）。在泪腺里。GERL位于邻近内高尔基体球囊，或由一个可变的距离从它分开。部分GERL常被修饰的粗面内质网池（RER）紧密包围，其邻近GERL的表面缺乏核糖体。分泌酶过氧化物酶的二氨基联苯胺反应产物定位于核膜的池，RER，外周高尔基囊泡，高尔基球囊，和未成熟和成熟的分泌颗粒。GERL通常不含过氧化物酶反应产物或仅含少量。硫胺素焦磷酸酶反应产物存在于两个到四个内部高尔基球囊，偶尔，最里面的球囊扩张和开窗，并含有较少的反应产物比下一个相邻球囊。酸性磷酸酶反应产物存在于GERL，不成熟的颗粒，很少，在最里面的球囊，但不是在其余的高尔基体球囊。在100 kV电压下观察的Acceptor制备的厚切片显示，GERL由脑池和有孔或管状部分组成。未成熟颗粒通过与小管部分的多个连接附着于GERL。这些结果表明，在大鼠眶外泪腺中，高尔基体囊参与分泌蛋白的运输，并且GERL参与分泌颗粒的形成。
The method of secretory granuleformation in the acinar cells of the rat exorbital lacrimal gland was studied by electron microscope morphological and cytochemical techniques. Immature secretory granules at the inner face of the Golgi apparatus were frequently attached to a narrow cisternal structure similar to GERL as described in neurons by Novikoff et al. (Novikoff, P. M., A. B. Novikoff, N. Quintana, and J.-J. Hauw. 1971. J. Cell Bio. 50:859-886). In the lacrimal gland. GERL was located adjacent to the inner Golgi saccule, or separated from it by a variable distance. Portions of GERL were often closely paralleled by modified cisternae of rough endoplasmic reticulum (RER), which lacked ribosomes on the surface adjacent to GERL. Diaminobenzidine reaction product of the secretory enzyme peroxidase was localized in the cisternae of the nuclear envelope, RER, peripheral Golgi vesicles, Golgi saccules, and immature and mature secretory granules. GERL was usually free of peroxidase reaction product or contained only a small amount. Thiamine pyrophosphatase reaction product was present in two to four inner Golgi saccules; occasionally, the innermost saccule was dilated and fenestrated, and contained less reaction product than the next adjacent saccule. Acid phosphatase (AcPase) reaction product was present in GERL, immature granules, and, rarely, in the innermost saccule, but not in the rest of the Golgi saccules. Thick sections of AcPase preparations viewed at 100 kV revealed that GERL consisted of cisternal, and fenestrated or tublular portions. The immature granules were attached to GERL by multiple connections to the tublular portions. These results suggest that, in the rat exorbital lacrimal gland, the Golgi saccules participate in the transport of secretory proteins, and that GERL is involved in the formation of secretory granules.